Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Toshihisa Nomura; Komaki Inoue; Yukiko Uehara-Yamaguchi; Koji Yamada; Osamu Iwata; Kengo Suzuki; Keiichi Mochida

doi:10.1111/pbi.13174

Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Toshihisa Nomura, Komaki Inoue, Yukiko Uehara-Yamaguchi, Koji Yamada, Osamu Iwata, Kengo Suzuki, Keiichi Mochida

Institute of Plant Science and Resources

Research output: Contribution to journal › Letter

1 Citation (Scopus)

Original language	English
Journal	Plant Biotechnology Journal
DOIs	https://doi.org/10.1111/pbi.13174
Publication status	Published - Jan 1 2019

Keywords

CRISPR/Cas9
Euglena gracilis
genome editing
knock-in
Microalgae
ribonucleoproteins
single-stranded oligodeoxynucleotides
transgene free

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Plant Science

Cite this

Nomura, T., Inoue, K., Uehara-Yamaguchi, Y., Yamada, K., Iwata, O., Suzuki, K., & Mochida, K. (2019). Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.13174

Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins. / Nomura, Toshihisa; Inoue, Komaki; Uehara-Yamaguchi, Yukiko; Yamada, Koji; Iwata, Osamu; Suzuki, Kengo; Mochida, Keiichi.

In: Plant Biotechnology Journal, 01.01.2019.

Research output: Contribution to journal › Letter

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AU - Inoue, Komaki

AU - Uehara-Yamaguchi, Yukiko

AU - Yamada, Koji

AU - Iwata, Osamu

AU - Suzuki, Kengo

AU - Mochida, Keiichi

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Access to Document

10.1111/pbi.13174