Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Toshihisa Nomura; Komaki Inoue; Yukiko Uehara-Yamaguchi; Koji Yamada; Osamu Iwata; Kengo Suzuki; Keiichi Mochida

doi:10.1111/pbi.13174

Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Toshihisa Nomura, Komaki Inoue, Yukiko Uehara-Yamaguchi, Koji Yamada, Osamu Iwata, Kengo Suzuki, Keiichi Mochida

Institute of Plant Science and Resources

Research output: Contribution to journal › Editorial

5 Citations (Scopus)

Original language	English
Pages (from-to)	2032-2034
Number of pages	3
Journal	Plant Biotechnology Journal
Volume	17
Issue number	11
DOIs	https://doi.org/10.1111/pbi.13174
Publication status	Published - Nov 1 2019

Keywords

CRISPR/Cas9
Euglena gracilis
Microalgae
genome editing
knock-in
ribonucleoproteins
single-stranded oligodeoxynucleotides
transgene free

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Plant Science

Access to Document

10.1111/pbi.13174

Cite this

Nomura, T., Inoue, K., Uehara-Yamaguchi, Y., Yamada, K., Iwata, O., Suzuki, K., & Mochida, K. (2019). Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins. Plant Biotechnology Journal, 17(11), 2032-2034. https://doi.org/10.1111/pbi.13174

Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins. / Nomura, Toshihisa; Inoue, Komaki; Uehara-Yamaguchi, Yukiko; Yamada, Koji; Iwata, Osamu; Suzuki, Kengo; Mochida, Keiichi.

In: Plant Biotechnology Journal, Vol. 17, No. 11, 01.11.2019, p. 2032-2034.

Research output: Contribution to journal › Editorial

Nomura, T, Inoue, K, Uehara-Yamaguchi, Y, Yamada, K, Iwata, O, Suzuki, K & Mochida, K 2019, 'Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins', Plant Biotechnology Journal, vol. 17, no. 11, pp. 2032-2034. https://doi.org/10.1111/pbi.13174

Nomura, Toshihisa ; Inoue, Komaki ; Uehara-Yamaguchi, Yukiko ; Yamada, Koji ; Iwata, Osamu ; Suzuki, Kengo ; Mochida, Keiichi. / Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins. In: Plant Biotechnology Journal. 2019 ; Vol. 17, No. 11. pp. 2032-2034.

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