Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Toshihisa Nomura, Komaki Inoue, Yukiko Uehara-Yamaguchi, Koji Yamada, Osamu Iwata, Kengo Suzuki, Keiichi Mochida

Research output: Contribution to journalLetter

1 Citation (Scopus)
Original languageEnglish
JournalPlant Biotechnology Journal
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • CRISPR/Cas9
  • Euglena gracilis
  • genome editing
  • knock-in
  • Microalgae
  • ribonucleoproteins
  • single-stranded oligodeoxynucleotides
  • transgene free

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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