Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Toshihisa Nomura, Komaki Inoue, Yukiko Uehara-Yamaguchi, Koji Yamada, Osamu Iwata, Kengo Suzuki, Keiichi Mochida

    Research output: Contribution to journalEditorialpeer-review

    11 Citations (Scopus)
    Original languageEnglish
    Pages (from-to)2032-2034
    Number of pages3
    JournalPlant Biotechnology Journal
    Issue number11
    Publication statusPublished - Nov 1 2019


    • CRISPR/Cas9
    • Euglena gracilis
    • Microalgae
    • genome editing
    • knock-in
    • ribonucleoproteins
    • single-stranded oligodeoxynucleotides
    • transgene free

    ASJC Scopus subject areas

    • Biotechnology
    • Agronomy and Crop Science
    • Plant Science

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