Highly efficient production of l-cystathionine from O-succinyl-l-homoserine and l-cysteine by Streptomyces cystathionine γ-lyase

Hiroshi Kanzaki, Toru Nagasawa, Hideaki Yamada

Research output: Contribution to journalArticle

Abstract

Utilizing the γ-replacement reaction of Streptomyces cystathionine γ-lyase (EC 4.4.1.1.), an efficient production method for l-cystathionine has been established. Under optimal conditions, 50 mM l-cystathionine was synthesized from 50 mM O-succinyl-l-homoserine and 50 mM l-cysteine, added in four stages to the reaction mixture, with a substrate conversion rate of 100%. This productivity (11 gl-1 of reaction mixture) is about 3.5 times higher than that with l-homoserine and l-cysteine as substrates.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalApplied Microbiology and Biotechnology
Volume25
Issue number2
DOIs
Publication statusPublished - Jan 1 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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