Higher amounts of anthocyanins and UV-absorbing compounds effectively lowered CPD photorepair in purple rice (Oryza sativa L.)

H. Hada, J. Hidema, Masahiko Maekawa, T. Kumagai

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Growth of a near-isogenic line (NIL) for the purple leaf gene Pl of rice with a genetic background of Taichung 65 (T-65) rice was significantly retarded by supplementary ultraviolet-B radiation (UV-B), despite the fact that the amounts of UV-absorbing compounds and anthocyanins in NIL were significantly higher than those in T-65. In order to understand the role of flavonoids in UV-B induced damage protection in T-65 and the NIL, both the (1) relationships between changes in the steady state of cyclobutane pyrimidine dimer (CPD) levels and changes in accumulation of anthocyanins and UV-absorbing compounds in leaves with leaf age, and (2) the susceptibility to CPD induction by UV-B radiation and the ability to photorepair CPD were examined. Although supplementary UV-B elevated the steady state of CPD levels in leaves in both strains, the level in the leaf of the NIL was higher than that in T-65 at any time. The susceptibility to CPD induction by short-term (challenge) UV-B exposure was lower in the NIL than in T-65. On the other hand, the CPD photorepair was also lower in the leaves of the NIL than in those of T-65. The decrease in CPD-photorepair in the NIL was due to a lowering of the leaf-penetrating blue/UV-A radiation, which is effective for photoreactivation by photolyase, by anthocyanins. Thus, accumulation of anthocyanins and UV-absorbing compounds did not effectively function as screening against damage caused by elevated UV-B radiation in the NIL, and the retardation of growth in the NIL resulted from its lower ability to photorepair CPD by higher amounts of anthocyanins.

Original languageEnglish
Pages (from-to)1691-1701
Number of pages11
JournalPlant, Cell and Environment
Volume26
Issue number10
DOIs
Publication statusPublished - Oct 2003

Fingerprint

Pyrimidine Dimers
Anthocyanins
pyrimidines
isogenic lines
anthocyanins
Oryza sativa
Radiation
rice
ultraviolet radiation
leaves
Deoxyribodipyrimidine Photo-Lyase
Oryza
Growth
Flavonoids
genetic background
growth retardation
flavonoids
screening

Keywords

  • Blue/UV-A radiation
  • Challenge exposure
  • Chronic exposure
  • Cyclobutane pyrimidine dimer (CPD)-photolyase
  • DNA damage
  • Near-isogenic line
  • Photoreactivation

ASJC Scopus subject areas

  • Plant Science

Cite this

Higher amounts of anthocyanins and UV-absorbing compounds effectively lowered CPD photorepair in purple rice (Oryza sativa L.). / Hada, H.; Hidema, J.; Maekawa, Masahiko; Kumagai, T.

In: Plant, Cell and Environment, Vol. 26, No. 10, 10.2003, p. 1691-1701.

Research output: Contribution to journalArticle

Hada, H. ; Hidema, J. ; Maekawa, Masahiko ; Kumagai, T. / Higher amounts of anthocyanins and UV-absorbing compounds effectively lowered CPD photorepair in purple rice (Oryza sativa L.). In: Plant, Cell and Environment. 2003 ; Vol. 26, No. 10. pp. 1691-1701.
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T1 - Higher amounts of anthocyanins and UV-absorbing compounds effectively lowered CPD photorepair in purple rice (Oryza sativa L.)

AU - Hada, H.

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AU - Maekawa, Masahiko

AU - Kumagai, T.

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AB - Growth of a near-isogenic line (NIL) for the purple leaf gene Pl of rice with a genetic background of Taichung 65 (T-65) rice was significantly retarded by supplementary ultraviolet-B radiation (UV-B), despite the fact that the amounts of UV-absorbing compounds and anthocyanins in NIL were significantly higher than those in T-65. In order to understand the role of flavonoids in UV-B induced damage protection in T-65 and the NIL, both the (1) relationships between changes in the steady state of cyclobutane pyrimidine dimer (CPD) levels and changes in accumulation of anthocyanins and UV-absorbing compounds in leaves with leaf age, and (2) the susceptibility to CPD induction by UV-B radiation and the ability to photorepair CPD were examined. Although supplementary UV-B elevated the steady state of CPD levels in leaves in both strains, the level in the leaf of the NIL was higher than that in T-65 at any time. The susceptibility to CPD induction by short-term (challenge) UV-B exposure was lower in the NIL than in T-65. On the other hand, the CPD photorepair was also lower in the leaves of the NIL than in those of T-65. The decrease in CPD-photorepair in the NIL was due to a lowering of the leaf-penetrating blue/UV-A radiation, which is effective for photoreactivation by photolyase, by anthocyanins. Thus, accumulation of anthocyanins and UV-absorbing compounds did not effectively function as screening against damage caused by elevated UV-B radiation in the NIL, and the retardation of growth in the NIL resulted from its lower ability to photorepair CPD by higher amounts of anthocyanins.

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