High-voltage electron microscopy reveals direct synaptic inputs from a spinal gastrin-releasing peptide system to neurons of the spinal nucleus of bulbocavernosus

Hirotaka Sakamoto, Tatsuo Arii, Mitsuhiro Kawata

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The spinal nucleus of bulbocavernosus (SNB) is a sexually dimorphic motor nucleus located in the anterior horn of the fifth and sixth lumbar segments of the spinal cord that plays a significant role in male sexual function. We recently found that a sexually dimorphic expression of gastrin-releasing peptide (GRP) in the lumbar spinal cord regulates male copulatory reflexes. Although it is reported that these systems are both profoundly regulated by circulating androgen levels in male rats, no direct evidence has been reported regarding GRP synaptic inputs onto SNB motoneurons. The aim of the current study was to determine the axodendritic synaptic inputs of spinal GRP neurons to SNB motoneurons. Immunoelectron microscopy, combined with a retrograde tracing technique using high-voltage electron microscopy (HVEM), provided a three-dimensional visualization of synaptic contacts from the GRP system in the lumbar spinal cord onto SNB motoneurons. HVEM analysis clearly demonstrated that GRP-immunoreactive axon terminals directly contact dendrites that extend into the dorsal gray commissure from the SNB. These HVEM findings provide an ultrastructural basis for understanding how the spinal GRP system regulates male sexual behavior.

Original languageEnglish
Pages (from-to)417-421
Number of pages5
JournalEndocrinology
Volume151
Issue number1
DOIs
Publication statusPublished - Jan 2010

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Gastrin-Releasing Peptide
Electron Microscopy
Spinal Cord
Neurons
Motor Neurons
Immunoelectron Microscopy
Presynaptic Terminals
Horns
Dendrites
Sexual Behavior
Androgens
Reflex

ASJC Scopus subject areas

  • Endocrinology

Cite this

High-voltage electron microscopy reveals direct synaptic inputs from a spinal gastrin-releasing peptide system to neurons of the spinal nucleus of bulbocavernosus. / Sakamoto, Hirotaka; Arii, Tatsuo; Kawata, Mitsuhiro.

In: Endocrinology, Vol. 151, No. 1, 01.2010, p. 417-421.

Research output: Contribution to journalArticle

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