High viral load of Merkel cell polyomavirus DNA sequences in Langerhans cell sarcoma tissues

Ichiro Murakami, Michiko Matsushita, Takeshi Iwasaki, Satoshi Kuwamoto, Masako Kato, Yasushi Horie, Kazuhiko Hayashi, Jean Gogusev, Francis Jaubert, Shu Nakamoto, Mitsunori Yamakawa, Hirokazu Nakamine, Katsuyoshi Takata, Takashi Oka, Tadashi Yoshino

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Abstract

Background: Langerhans cell (LC) sarcoma (LCS) is a high-grade neoplasm with overtly malignant cytologic features and an LC phenotype. We very recently suggested that LC behaves as a reservoir for common dermotropic Merkel cell polyomavirus (MCPyV) and determined the relationship between LC histiocytosis (LCH), which has an underlining oncogenic capacity, and MCPyV as a trigger for a reactive process rather than a neoplastic process. We propose LC to be a reservoir for MCPyV and hypothesize that some LCS subtypes may be related to the MCPyV agent. Findings. We examined seven LCS tissues using multiplex quantitative PCR (Q-PCR) and immunohistochemistry with anti MCPyV large-T (LT) antigen antibody. High viral loads of MCPyV DNA sequences (viral load = relative levels of MCPyV) were detected (0.328-0.772 copies/cell (Merkel cell carcinoma (MCC) = 1.0)) using Q-PCR in 43% (3/7) tissues, but LT antigen expression was not observed (0/7). Conclusions: Frequent MCPyV-DNA amplification suggests that LCS in some patients may be related to MCPyV infection. Moreover, the higher viral load of LCS (median, 0.453 copies/cell) than low load of LCH (0.003, median of 12 cases) (P <0.01) may suggest a virally induced tumorigenic process in some LCS. Although the absence of LT antigen expression may indicate a different role for MCPyV in this pathology, some subtypes of LCS may develop in the background of MCPyV-infected LC. To the best of our knowledge, this is the first report on the relationship between MCPyV and LCS. The recent discovery of MCPyV opened new therapeutic avenues for MCC. These data open novel possibilities for therapeutic interventions against LCS.

Original languageEnglish
Article number15
JournalInfectious Agents and Cancer
Volume9
Issue number1
DOIs
Publication statusPublished - May 6 2014

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Langerhans Cell Sarcoma
Merkel cell polyomavirus
Viral Load
Sarcoma
Langerhans Cells
Merkel Cell Carcinoma
Viral Tumor Antigens
Polyomavirus Infections
Histiocytosis
Polyomavirus Transforming Antigens
Neoplastic Processes
Langerhans Cell Histiocytosis

Keywords

  • Langerhans cell
  • Langerhans cell sarcoma
  • Merkel cell polyomavirus
  • Multiplex quantitative PCR

ASJC Scopus subject areas

  • Infectious Diseases
  • Oncology
  • Epidemiology
  • Cancer Research

Cite this

High viral load of Merkel cell polyomavirus DNA sequences in Langerhans cell sarcoma tissues. / Murakami, Ichiro; Matsushita, Michiko; Iwasaki, Takeshi; Kuwamoto, Satoshi; Kato, Masako; Horie, Yasushi; Hayashi, Kazuhiko; Gogusev, Jean; Jaubert, Francis; Nakamoto, Shu; Yamakawa, Mitsunori; Nakamine, Hirokazu; Takata, Katsuyoshi; Oka, Takashi; Yoshino, Tadashi.

In: Infectious Agents and Cancer, Vol. 9, No. 1, 15, 06.05.2014.

Research output: Contribution to journalArticle

Murakami, I, Matsushita, M, Iwasaki, T, Kuwamoto, S, Kato, M, Horie, Y, Hayashi, K, Gogusev, J, Jaubert, F, Nakamoto, S, Yamakawa, M, Nakamine, H, Takata, K, Oka, T & Yoshino, T 2014, 'High viral load of Merkel cell polyomavirus DNA sequences in Langerhans cell sarcoma tissues', Infectious Agents and Cancer, vol. 9, no. 1, 15. https://doi.org/10.1186/1750-9378-9-15
Murakami, Ichiro ; Matsushita, Michiko ; Iwasaki, Takeshi ; Kuwamoto, Satoshi ; Kato, Masako ; Horie, Yasushi ; Hayashi, Kazuhiko ; Gogusev, Jean ; Jaubert, Francis ; Nakamoto, Shu ; Yamakawa, Mitsunori ; Nakamine, Hirokazu ; Takata, Katsuyoshi ; Oka, Takashi ; Yoshino, Tadashi. / High viral load of Merkel cell polyomavirus DNA sequences in Langerhans cell sarcoma tissues. In: Infectious Agents and Cancer. 2014 ; Vol. 9, No. 1.
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abstract = "Background: Langerhans cell (LC) sarcoma (LCS) is a high-grade neoplasm with overtly malignant cytologic features and an LC phenotype. We very recently suggested that LC behaves as a reservoir for common dermotropic Merkel cell polyomavirus (MCPyV) and determined the relationship between LC histiocytosis (LCH), which has an underlining oncogenic capacity, and MCPyV as a trigger for a reactive process rather than a neoplastic process. We propose LC to be a reservoir for MCPyV and hypothesize that some LCS subtypes may be related to the MCPyV agent. Findings. We examined seven LCS tissues using multiplex quantitative PCR (Q-PCR) and immunohistochemistry with anti MCPyV large-T (LT) antigen antibody. High viral loads of MCPyV DNA sequences (viral load = relative levels of MCPyV) were detected (0.328-0.772 copies/cell (Merkel cell carcinoma (MCC) = 1.0)) using Q-PCR in 43{\%} (3/7) tissues, but LT antigen expression was not observed (0/7). Conclusions: Frequent MCPyV-DNA amplification suggests that LCS in some patients may be related to MCPyV infection. Moreover, the higher viral load of LCS (median, 0.453 copies/cell) than low load of LCH (0.003, median of 12 cases) (P <0.01) may suggest a virally induced tumorigenic process in some LCS. Although the absence of LT antigen expression may indicate a different role for MCPyV in this pathology, some subtypes of LCS may develop in the background of MCPyV-infected LC. To the best of our knowledge, this is the first report on the relationship between MCPyV and LCS. The recent discovery of MCPyV opened new therapeutic avenues for MCC. These data open novel possibilities for therapeutic interventions against LCS.",
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AU - Iwasaki, Takeshi

AU - Kuwamoto, Satoshi

AU - Kato, Masako

AU - Horie, Yasushi

AU - Hayashi, Kazuhiko

AU - Gogusev, Jean

AU - Jaubert, Francis

AU - Nakamoto, Shu

AU - Yamakawa, Mitsunori

AU - Nakamine, Hirokazu

AU - Takata, Katsuyoshi

AU - Oka, Takashi

AU - Yoshino, Tadashi

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N2 - Background: Langerhans cell (LC) sarcoma (LCS) is a high-grade neoplasm with overtly malignant cytologic features and an LC phenotype. We very recently suggested that LC behaves as a reservoir for common dermotropic Merkel cell polyomavirus (MCPyV) and determined the relationship between LC histiocytosis (LCH), which has an underlining oncogenic capacity, and MCPyV as a trigger for a reactive process rather than a neoplastic process. We propose LC to be a reservoir for MCPyV and hypothesize that some LCS subtypes may be related to the MCPyV agent. Findings. We examined seven LCS tissues using multiplex quantitative PCR (Q-PCR) and immunohistochemistry with anti MCPyV large-T (LT) antigen antibody. High viral loads of MCPyV DNA sequences (viral load = relative levels of MCPyV) were detected (0.328-0.772 copies/cell (Merkel cell carcinoma (MCC) = 1.0)) using Q-PCR in 43% (3/7) tissues, but LT antigen expression was not observed (0/7). Conclusions: Frequent MCPyV-DNA amplification suggests that LCS in some patients may be related to MCPyV infection. Moreover, the higher viral load of LCS (median, 0.453 copies/cell) than low load of LCH (0.003, median of 12 cases) (P <0.01) may suggest a virally induced tumorigenic process in some LCS. Although the absence of LT antigen expression may indicate a different role for MCPyV in this pathology, some subtypes of LCS may develop in the background of MCPyV-infected LC. To the best of our knowledge, this is the first report on the relationship between MCPyV and LCS. The recent discovery of MCPyV opened new therapeutic avenues for MCC. These data open novel possibilities for therapeutic interventions against LCS.

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