High temperature causes breakdown of S haplotype-dependent stigmatic self-incompatibility in self-incompatible Arabidopsis thaliana

Masaya Yamamoto, Kenji Nishimura, Hiroyasu Kitashiba, Wataru Sakamoto, Takeshi Nishio

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Commercial seeds of Brassicaceae vegetable crops are mostly F1 hybrids, the production of which depends on self-incompatibility during pollination. Self-incompatibility is known to be weakened by exposure to elevated temperatures, which may compromise future breeding and seed production. In the Brassicaceae, self-incompatibility is controlled by two genes, SRK and SCR, which function as female and male determinants of recognition specificity, respectively. However, the molecular mechanisms underlying the breakdown of self-incompatibility under high temperature are poorly understood. In this study, we examined the self-incompatibility phenotypes of self-incompatible Arabidopsis thaliana SRK-SCR transformants under normal (23 C) and elevated (29 C) temperatures. Exposure to elevated temperature caused defects in the stigmatic, but not the pollen, self-incompatibility response. In addition, differences in the response to elevated temperature were observed among different S haplotypes. Subcellular localization revealed that high temperature disrupted the targeting of SRK to the plasma membrane. SRK localization in plants transformed with different S haplotypes corresponded to their self-incompatibility phenotypes, further indicating that defects in SRK localization were responsible for the breakdown in the self-incompatibility response at high temperature. Our results provide new insights into the causes of instability in self-incompatibility phenotypes.

    Original languageEnglish
    Pages (from-to)5745-5751
    Number of pages7
    JournalJournal of experimental botany
    Volume70
    Issue number20
    DOIs
    Publication statusPublished - Oct 24 2019

    Keywords

    • Brassicaceae
    • F hybrid
    • high temperature
    • protein transporting
    • receptor kinase
    • self-incompatibility

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

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