High sensitivity of RBL-2H3 cells to cadmium and manganese

An implication of the role of ZIP8

Hitomi Fujishiro, Miwako Doi, Shuichi Enomoto, Seiichiro Himeno

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Cellular incorporation of Cd involves multiple transport systems for other metals such as Fe, Zn, Mn, and Ca. Metal transporters including divalent metal transporter 1, Zrt/Irt-related protein (ZIP) 8, and ZIP14, and certain types of voltage-dependent Ca channels have been shown to be involved in cellular Cd uptake. However, tissue- or cell-specific roles of these metal transporters in the accumulation and toxicity of Cd remains unclear. In the present study, we compared the sensitivity to and accumulation of Cd, Mn, and Zn among four types of rat cell lines. Rat basophilic leukemia RBL-2H3 cells showed the highest sensitivity to Cd and Mn due to the highest accumulation of Cd and Mn among the four cell lines. The high accumulation of Cd and Mn was caused by high uptake rates of Cd and Mn. Since relatively high expression of ZIP8 and ZIP14 was found in RBL-2H3 cells, siRNAs of ZIP8 and ZIP14 were transfected into RBL-2H3 cells. The knockdown of ZIP8, but not of ZIP14, significantly reduced the uptake rates of Cd and Mn in RBL-2H3 cells, especially in the presence of bicarbonate. These results suggest that the high expression of ZIP8, which is known to have affinities for both Cd and Mn, resulted in high accumulation of Cd and Mn, leading to high sensitivity to these metals in RBL-2H3 cells. Thus, RBL-2H3 cells may serve as a good model for clarifying the mechanisms of Cd and Mn transport via ZIP8.

Original languageEnglish
Pages (from-to)710-718
Number of pages9
JournalMetallomics
Volume3
Issue number7
DOIs
Publication statusPublished - Jul 2011

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Manganese
Cadmium
Metals
Rats
Cells
Bicarbonates
Toxicity
Cell Line
Tissue
Electric potential
Proteins
Leukemia

ASJC Scopus subject areas

  • Biomaterials
  • Metals and Alloys
  • Chemistry (miscellaneous)
  • Biochemistry
  • Biophysics

Cite this

High sensitivity of RBL-2H3 cells to cadmium and manganese : An implication of the role of ZIP8. / Fujishiro, Hitomi; Doi, Miwako; Enomoto, Shuichi; Himeno, Seiichiro.

In: Metallomics, Vol. 3, No. 7, 07.2011, p. 710-718.

Research output: Contribution to journalArticle

Fujishiro, Hitomi ; Doi, Miwako ; Enomoto, Shuichi ; Himeno, Seiichiro. / High sensitivity of RBL-2H3 cells to cadmium and manganese : An implication of the role of ZIP8. In: Metallomics. 2011 ; Vol. 3, No. 7. pp. 710-718.
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