Abstract
In order to determine micoamounts of proteins with low molecular diffusivity, a flow method was developed by both miniaturizing the common flow-injection apparatus and using the dye association method. A very precise double-plunger micropump, which was recently developed for this purpose, was used. The optimum conditions using bovine serum albumin (BSA) as standard and some ion-association dye reagents at very low flow-rates were examined. It was shown that a flow-rate of less than 50 μl min-1 was acceptable when a short, small-bore reaction tube was used. Up to 15 samples H-1 could be analysed with a relatively long residence time; linear calibration graphs were obtained with both 8-μl volume loop valve injection and no-injection methods. The detection limit and relative standard deviation of the injection method were 0.003 μg ml-1 and 0.15%, respectively, for BSA.
Original language | English |
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Pages (from-to) | 67-74 |
Number of pages | 8 |
Journal | Analytica Chimica Acta |
Volume | 261 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - May 25 1992 |
Keywords
- Biological samples
- Flow system
- Proteins
- Spectrophotometry
- Urine
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy