High-sensitivity flow method for the determination of proteins with micro-flow plunger pumps

Takashi Korenaga, Xiaojing Zhou, Miyoko Izawa, Teruo Takahashi, Tosio Moriwake, Sumio Shinoda

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


In order to determine micoamounts of proteins with low molecular diffusivity, a flow method was developed by both miniaturizing the common flow-injection apparatus and using the dye association method. A very precise double-plunger micropump, which was recently developed for this purpose, was used. The optimum conditions using bovine serum albumin (BSA) as standard and some ion-association dye reagents at very low flow-rates were examined. It was shown that a flow-rate of less than 50 μl min-1 was acceptable when a short, small-bore reaction tube was used. Up to 15 samples H-1 could be analysed with a relatively long residence time; linear calibration graphs were obtained with both 8-μl volume loop valve injection and no-injection methods. The detection limit and relative standard deviation of the injection method were 0.003 μg ml-1 and 0.15%, respectively, for BSA.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalAnalytica Chimica Acta
Issue number1-2
Publication statusPublished - May 25 1992


  • Biological samples
  • Flow system
  • Proteins
  • Spectrophotometry
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy


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