High-sensitivity flow method for the determination of proteins with micro-flow plunger pumps

Takashi Korenaga, Xiaojing Zhou, Miyoko Izawa, Teruo Takahashi, Tosio Moriwake, Sumio Shinoda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In order to determine micoamounts of proteins with low molecular diffusivity, a flow method was developed by both miniaturizing the common flow-injection apparatus and using the dye association method. A very precise double-plunger micropump, which was recently developed for this purpose, was used. The optimum conditions using bovine serum albumin (BSA) as standard and some ion-association dye reagents at very low flow-rates were examined. It was shown that a flow-rate of less than 50 μl min-1 was acceptable when a short, small-bore reaction tube was used. Up to 15 samples H-1 could be analysed with a relatively long residence time; linear calibration graphs were obtained with both 8-μl volume loop valve injection and no-injection methods. The detection limit and relative standard deviation of the injection method were 0.003 μg ml-1 and 0.15%, respectively, for BSA.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalAnalytica Chimica Acta
Volume261
Issue number1-2
DOIs
Publication statusPublished - May 25 1992

Fingerprint

Bovine Serum Albumin
pump
Coloring Agents
Flow rate
Pumps
Injections
protein
serum
dye
Proteins
Calibration
Ions
low flow
diffusivity
Limit of Detection
residence time
calibration
method
ion
bovine

Keywords

  • Biological samples
  • Flow system
  • Proteins
  • Spectrophotometry
  • Urine

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Korenaga, T., Zhou, X., Izawa, M., Takahashi, T., Moriwake, T., & Shinoda, S. (1992). High-sensitivity flow method for the determination of proteins with micro-flow plunger pumps. Analytica Chimica Acta, 261(1-2), 67-74. https://doi.org/10.1016/0003-2670(92)80177-9

High-sensitivity flow method for the determination of proteins with micro-flow plunger pumps. / Korenaga, Takashi; Zhou, Xiaojing; Izawa, Miyoko; Takahashi, Teruo; Moriwake, Tosio; Shinoda, Sumio.

In: Analytica Chimica Acta, Vol. 261, No. 1-2, 25.05.1992, p. 67-74.

Research output: Contribution to journalArticle

Korenaga, T, Zhou, X, Izawa, M, Takahashi, T, Moriwake, T & Shinoda, S 1992, 'High-sensitivity flow method for the determination of proteins with micro-flow plunger pumps', Analytica Chimica Acta, vol. 261, no. 1-2, pp. 67-74. https://doi.org/10.1016/0003-2670(92)80177-9
Korenaga, Takashi ; Zhou, Xiaojing ; Izawa, Miyoko ; Takahashi, Teruo ; Moriwake, Tosio ; Shinoda, Sumio. / High-sensitivity flow method for the determination of proteins with micro-flow plunger pumps. In: Analytica Chimica Acta. 1992 ; Vol. 261, No. 1-2. pp. 67-74.
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