TY - JOUR
T1 - High-sensitivity flow method for the determination of proteins with micro-flow plunger pumps
AU - Korenaga, Takashi
AU - Zhou, Xiaojing
AU - Izawa, Miyoko
AU - Takahashi, Teruo
AU - Moriwake, Tosio
AU - Shinoda, Sumio
N1 - Funding Information:
One of the authors (T K) thanks the Mmlstry of Education, Science and Culture, Japan, for partial financial support
PY - 1992/5/25
Y1 - 1992/5/25
N2 - In order to determine micoamounts of proteins with low molecular diffusivity, a flow method was developed by both miniaturizing the common flow-injection apparatus and using the dye association method. A very precise double-plunger micropump, which was recently developed for this purpose, was used. The optimum conditions using bovine serum albumin (BSA) as standard and some ion-association dye reagents at very low flow-rates were examined. It was shown that a flow-rate of less than 50 μl min-1 was acceptable when a short, small-bore reaction tube was used. Up to 15 samples H-1 could be analysed with a relatively long residence time; linear calibration graphs were obtained with both 8-μl volume loop valve injection and no-injection methods. The detection limit and relative standard deviation of the injection method were 0.003 μg ml-1 and 0.15%, respectively, for BSA.
AB - In order to determine micoamounts of proteins with low molecular diffusivity, a flow method was developed by both miniaturizing the common flow-injection apparatus and using the dye association method. A very precise double-plunger micropump, which was recently developed for this purpose, was used. The optimum conditions using bovine serum albumin (BSA) as standard and some ion-association dye reagents at very low flow-rates were examined. It was shown that a flow-rate of less than 50 μl min-1 was acceptable when a short, small-bore reaction tube was used. Up to 15 samples H-1 could be analysed with a relatively long residence time; linear calibration graphs were obtained with both 8-μl volume loop valve injection and no-injection methods. The detection limit and relative standard deviation of the injection method were 0.003 μg ml-1 and 0.15%, respectively, for BSA.
KW - Biological samples
KW - Flow system
KW - Proteins
KW - Spectrophotometry
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=0026550753&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026550753&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(92)80177-9
DO - 10.1016/0003-2670(92)80177-9
M3 - Article
AN - SCOPUS:0026550753
VL - 261
SP - 67
EP - 74
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1-2
ER -