Abstract
Background/Aims: Glomerular basement membranes (GBM) and tubular basement membranes (TBM) consist of a fine meshwork composed mainly of type IV collagen. Each segment of tubules has specialized physiologic functions, and thus we investigated the ultrastructure of various basement membranes in rat kidneys. Methods: Since purifying basement membranes from different tubule segments is technically challenging, we employed tissue negative staining rather than conventional negative staining to compare the ultrastructures of proximal and distal TBM and GBM in normal rats. We also assessed the distribution of extracellular matrix components including type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin in the basement membranes by immunohistochemistry. Results: TBM and GBM of normal rats showed a fine meshwork structure consisting of fibrils forming small round to oval pores. Short- and long-pore diameters in proximal tubules were 3.3 ± 0.5 and 3.9 ± 0.6 nm, respectively, and in distal tubules 3.5 ± 0.7 and 4.3 ± 0.8 nm, respectively. For GBM the respective diameters were 2.5 ± 0.5 and 3.0 ± 0.5 nm. Immunohistochemical analysis showed no significant difference in distribution of extracellular matrix components between proximal and distal TBM. However, immunofluorescence scores of α1 chain of type IV collagen, fibronectin, and laminin were higher in the TBM than in the GBM. On the other hand, heparan sulfate proteoglycan was higher in the GBM. Conclusion: Ultrastructural differences in renal basement membranes may be related to differences in physiologic function in each segment.
| Original language | English |
|---|---|
| Pages (from-to) | 686-693 |
| Number of pages | 8 |
| Journal | American Journal of Nephrology |
| Volume | 19 |
| Issue number | 6 |
| Publication status | Published - Nov 1999 |
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Keywords
- Glomerular basement membrane
- Renal ultrastructure
- Tissue negative staining
- Tubular basement membrane
ASJC Scopus subject areas
- Nephrology
Cite this
High-resolution ultrastructural comparison of renal glomerular and tubular basement membranes. / Ogawa, Saeko; Ota, Zensuke; Shikata, Kenichi; Hironaka, Kazue; Hayashi, Yoshiko; Ota, Kosuke; Kushiro, Masahiko; Miyatake, Nobuyuki; Kishimoto, Noboru; Makino, Hirofumi.
In: American Journal of Nephrology, Vol. 19, No. 6, 11.1999, p. 686-693.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-resolution ultrastructural comparison of renal glomerular and tubular basement membranes
AU - Ogawa, Saeko
AU - Ota, Zensuke
AU - Shikata, Kenichi
AU - Hironaka, Kazue
AU - Hayashi, Yoshiko
AU - Ota, Kosuke
AU - Kushiro, Masahiko
AU - Miyatake, Nobuyuki
AU - Kishimoto, Noboru
AU - Makino, Hirofumi
PY - 1999/11
Y1 - 1999/11
N2 - Background/Aims: Glomerular basement membranes (GBM) and tubular basement membranes (TBM) consist of a fine meshwork composed mainly of type IV collagen. Each segment of tubules has specialized physiologic functions, and thus we investigated the ultrastructure of various basement membranes in rat kidneys. Methods: Since purifying basement membranes from different tubule segments is technically challenging, we employed tissue negative staining rather than conventional negative staining to compare the ultrastructures of proximal and distal TBM and GBM in normal rats. We also assessed the distribution of extracellular matrix components including type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin in the basement membranes by immunohistochemistry. Results: TBM and GBM of normal rats showed a fine meshwork structure consisting of fibrils forming small round to oval pores. Short- and long-pore diameters in proximal tubules were 3.3 ± 0.5 and 3.9 ± 0.6 nm, respectively, and in distal tubules 3.5 ± 0.7 and 4.3 ± 0.8 nm, respectively. For GBM the respective diameters were 2.5 ± 0.5 and 3.0 ± 0.5 nm. Immunohistochemical analysis showed no significant difference in distribution of extracellular matrix components between proximal and distal TBM. However, immunofluorescence scores of α1 chain of type IV collagen, fibronectin, and laminin were higher in the TBM than in the GBM. On the other hand, heparan sulfate proteoglycan was higher in the GBM. Conclusion: Ultrastructural differences in renal basement membranes may be related to differences in physiologic function in each segment.
AB - Background/Aims: Glomerular basement membranes (GBM) and tubular basement membranes (TBM) consist of a fine meshwork composed mainly of type IV collagen. Each segment of tubules has specialized physiologic functions, and thus we investigated the ultrastructure of various basement membranes in rat kidneys. Methods: Since purifying basement membranes from different tubule segments is technically challenging, we employed tissue negative staining rather than conventional negative staining to compare the ultrastructures of proximal and distal TBM and GBM in normal rats. We also assessed the distribution of extracellular matrix components including type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin in the basement membranes by immunohistochemistry. Results: TBM and GBM of normal rats showed a fine meshwork structure consisting of fibrils forming small round to oval pores. Short- and long-pore diameters in proximal tubules were 3.3 ± 0.5 and 3.9 ± 0.6 nm, respectively, and in distal tubules 3.5 ± 0.7 and 4.3 ± 0.8 nm, respectively. For GBM the respective diameters were 2.5 ± 0.5 and 3.0 ± 0.5 nm. Immunohistochemical analysis showed no significant difference in distribution of extracellular matrix components between proximal and distal TBM. However, immunofluorescence scores of α1 chain of type IV collagen, fibronectin, and laminin were higher in the TBM than in the GBM. On the other hand, heparan sulfate proteoglycan was higher in the GBM. Conclusion: Ultrastructural differences in renal basement membranes may be related to differences in physiologic function in each segment.
KW - Glomerular basement membrane
KW - Renal ultrastructure
KW - Tissue negative staining
KW - Tubular basement membrane
UR - http://www.scopus.com/inward/record.url?scp=0032727848&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032727848&partnerID=8YFLogxK
M3 - Article
VL - 19
SP - 686
EP - 693
JO - American Journal of Nephrology
JF - American Journal of Nephrology
SN - 0250-8095
IS - 6
ER -