High resolution three-dimensional meshwork structure of the glomerular basement membrane

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3 Citations (Scopus)

Abstract

To investigate the ultrastructure of the glomerular basement membrane (GBM), rat GBM was treated with sodium dodecyl sulfate and 2-mercaptoethanol and observed under an electron microscope employing ultrathin sectioning and rotary shadowing methods. Further, thick sections of the treated GBM were examined by high voltage transmission electron microscopy. A fine three-dimensional meshwork structure was clearly observed through the entire thickness of the GBM treated with sodium dodecyl sulfate and 2-mercaptoethanol by conventional transmission electron microscopy and high voltage transmission electron microscopy. The diameter of the fibrils forming the meshwork structure was about 3 nm and the dimensions of the pores present in the meshwork were 3 to 4 nm. The rotary shadowing technique revealed fine fibrils disentangled from the GBM that were bound together, and corresponded morphologically type IV collagen molecules. The present findings suggested that the GBM has a fine three-dimensional meshwork structure through its entire thickness which is composed mainly of type IV collagen and may function as a size barrier in the renal glomerulus.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
Journalthe japanese journal of nephrology
Volume34
Issue number4
DOIs
Publication statusPublished - Jan 1 1992

Keywords

  • glomerular basement membrane
  • high voltage electron microscopy
  • meshwork structure
  • rotary shadowing
  • type IV collagen

ASJC Scopus subject areas

  • Nephrology

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