High-resolution secondary ion mass spectrometry reveals the contrasting subcellular distribution of arsenic and silicon in rice roots

Katie L. Moore, Markus Schröder, Zhongchang Wu, Barry G H Martin, Chris R. Hawes, Steve P. McGrath, Malcolm J. Hawkesford, Jian Feng Ma, Fang Jie Zhao, Chris R M Grovenor

Research output: Contribution to journalArticle

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Abstract

Rice (Oryza sativa) takes up arsenite mainly through the silicic acid transport pathway. Understanding the uptake and sequestration of arsenic (As) into the rice plant is important for developing strategies to reduce As concentration in rice grain. In this study, the cellular and subcellular distributions of As and silicon (Si) in rice roots were investigated using high-pressure freezing, high-resolution secondary ion mass spectrometry, and transmission electron microscopy. Rice plants, both the lsi2 mutant lacking the Si/arsenite efflux transporter Lsi2 and its wild-type cultivar, with or without an iron plaque, were treated with arsenate or arsenite. The formation of iron plaque on the root surface resulted in strong accumulation of As and phosphorous on the epidermis. The lsi2 mutant showed stronger As accumulation in the endodermal vacuoles, where the Lsi2 transporter is located in the plasma membranes, than the wild-type line. As also accumulated in the vacuoles of some xylem parenchyma cells and in some pericycle cells, particularly in the wild-type mature root zone. Vacuolar accumulation of As is associated with sulfur, suggesting that As may be stored as arsenite-phytochelatin complexes. Si was localized in the cell walls of the endodermal cells with little apparent effect of the Lsi2 mutation on its distribution. This study reveals the vacuolar sequestration of As in rice roots and contrasting patterns of As and Si subcellular localization, despite both being transported across the plasma membranes by the same transporters.

Original languageEnglish
Pages (from-to)913-924
Number of pages12
JournalPlant Physiology
Volume156
Issue number2
DOIs
Publication statusPublished - Jun 2011

Fingerprint

Secondary Ion Mass Spectrometry
Arsenic
Silicon
silicon
arsenic
mass spectrometry
ions
rice
arsenites
transporters
Vacuoles
vacuoles
plasma membrane
Phytochelatins
Iron
Cell Membrane
Oryza
Silicic Acid
iron
Xylem

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Moore, K. L., Schröder, M., Wu, Z., Martin, B. G. H., Hawes, C. R., McGrath, S. P., ... Grovenor, C. R. M. (2011). High-resolution secondary ion mass spectrometry reveals the contrasting subcellular distribution of arsenic and silicon in rice roots. Plant Physiology, 156(2), 913-924. https://doi.org/10.1104/pp.111.173088

High-resolution secondary ion mass spectrometry reveals the contrasting subcellular distribution of arsenic and silicon in rice roots. / Moore, Katie L.; Schröder, Markus; Wu, Zhongchang; Martin, Barry G H; Hawes, Chris R.; McGrath, Steve P.; Hawkesford, Malcolm J.; Ma, Jian Feng; Zhao, Fang Jie; Grovenor, Chris R M.

In: Plant Physiology, Vol. 156, No. 2, 06.2011, p. 913-924.

Research output: Contribution to journalArticle

Moore, KL, Schröder, M, Wu, Z, Martin, BGH, Hawes, CR, McGrath, SP, Hawkesford, MJ, Ma, JF, Zhao, FJ & Grovenor, CRM 2011, 'High-resolution secondary ion mass spectrometry reveals the contrasting subcellular distribution of arsenic and silicon in rice roots', Plant Physiology, vol. 156, no. 2, pp. 913-924. https://doi.org/10.1104/pp.111.173088
Moore, Katie L. ; Schröder, Markus ; Wu, Zhongchang ; Martin, Barry G H ; Hawes, Chris R. ; McGrath, Steve P. ; Hawkesford, Malcolm J. ; Ma, Jian Feng ; Zhao, Fang Jie ; Grovenor, Chris R M. / High-resolution secondary ion mass spectrometry reveals the contrasting subcellular distribution of arsenic and silicon in rice roots. In: Plant Physiology. 2011 ; Vol. 156, No. 2. pp. 913-924.
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