High-resolution mapping of the nud locus controlling the naked caryopsis in barley

Shin Taketa, T. Awayama, S. Amano, Y. Sakurai, M. Ichii

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The hulled or naked caryopsis character of barley is an important agronomic trait because of the direct link to its use. A single recessive gene, nud, located on the long arm of chromosome 7H, controls the naked caryopsis character. Previously, linked amplified fragment length polymorphism (AFLP) bands from bulked segregant analysis were screened, and the nud gene was mapped in a population of 151 F2 plants. In the present study, the aim was to construct a high-resolution map of the nud gene towards its positional cloning. Two AFLP bands were converted into sequence-characterized amplified region (SCAR) markers (sKT5 and sKT9), and a previously reported SCAR marker sKT3 was improved for more reliable detection of polymorphism. A total of 2380 segregants derived from five cross-combinations were analysed, and the nud gene was flanked by sKT3 and sKT9, at the 0.6-cM proximal and the 0.06-cM distal side, respectively. The SCAR markers developed in this study should be useful for marker-assisted selection in naked barley breeding employing crosses between naked and hulled accessions.

Original languageEnglish
Pages (from-to)337-342
Number of pages6
JournalPlant Breeding
Volume125
Issue number4
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

Fingerprint

Hordeum
barley
loci
amplified fragment length polymorphism
fruits
Genes
Recessive Genes
genes
recessive genes
marker-assisted selection
agronomic traits
Breeding
Organism Cloning
molecular cloning
Chromosomes
genetic polymorphism
chromosomes
Population

Keywords

  • AFLP
  • Hordeum vulgare
  • Marker-assisted selection
  • Naked barley
  • Pooled sampling
  • SCAR marker

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Biotechnology

Cite this

High-resolution mapping of the nud locus controlling the naked caryopsis in barley. / Taketa, Shin; Awayama, T.; Amano, S.; Sakurai, Y.; Ichii, M.

In: Plant Breeding, Vol. 125, No. 4, 08.2006, p. 337-342.

Research output: Contribution to journalArticle

Taketa, Shin ; Awayama, T. ; Amano, S. ; Sakurai, Y. ; Ichii, M. / High-resolution mapping of the nud locus controlling the naked caryopsis in barley. In: Plant Breeding. 2006 ; Vol. 125, No. 4. pp. 337-342.
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