High-resolution immuno-scanning electron microscopy using a non-coating method: Study of herpes simplex virus glycoproteins on the surface of virus particles and infected cells

Jorge A. Padilla, Fumio Uno, Masao Yamada, Hikaru Nanba, Shiro Nii

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Abstract

The expression of two glycoproteins, i.e. glycoprotein C (gC) and glycoprotein D (gD), of herpes simplex virus type 1 (HSV-1) on the surface of extracellular particles of this virus was examined by immuno-scanning electron microscopy. Scanning electron microscopy specimens of infected cells immuno-labelled against the glycoproteins with colloidal gold particles were prepared by a conventional coating and a non-coating method. Surface ultrastructure of infected cells and gold particles were observed more clearly with specimens prepared by the non-coating method. The appearance of virus particles in association with glycoprotein expression on these particles and on the surface of infected cells was then studied. Progeny virus particles began to appear 6 h after infection, increased in number as the infection proceeded, and covered most of the cell surface by 16 h. Six to 24 h after the infection, the labelling density for each glycoprotein on virus particles remained constant. The labelling density for gD was always higher than that for gC. The patch-like distribution of gold-labelling against gD was often detected on infected cell monolayers at the exponential and late stage of one cycle of virus growth. The labelling density for gD on virus particles was the highest on these produced in Vero and L-929 cells, moderate in MRC-5, BHK-21 and FL cells, and the lowest in HEp-2 cells.

Original languageEnglish
Pages (from-to)171-180
Number of pages10
JournalJournal of Electron Microscopy
Volume46
Issue number2
Publication statusPublished - 1997

Fingerprint

Glycoproteins
Immunoelectron Microscopy
Membrane Glycoproteins
viruses
Simplexvirus
Viruses
Virion
Scanning electron microscopy
scanning electron microscopy
high resolution
cells
marking
infectious diseases
Labeling
gold
Gold
Infection
Gold Colloid
progeny
Human Herpesvirus 1

Keywords

  • Glycoprotein C
  • Glycoprotein D
  • Herpes simplex virus
  • Immuno-gold-labelling
  • Non-coating method
  • Ultrahigh-resolution SEM

ASJC Scopus subject areas

  • Instrumentation

Cite this

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abstract = "The expression of two glycoproteins, i.e. glycoprotein C (gC) and glycoprotein D (gD), of herpes simplex virus type 1 (HSV-1) on the surface of extracellular particles of this virus was examined by immuno-scanning electron microscopy. Scanning electron microscopy specimens of infected cells immuno-labelled against the glycoproteins with colloidal gold particles were prepared by a conventional coating and a non-coating method. Surface ultrastructure of infected cells and gold particles were observed more clearly with specimens prepared by the non-coating method. The appearance of virus particles in association with glycoprotein expression on these particles and on the surface of infected cells was then studied. Progeny virus particles began to appear 6 h after infection, increased in number as the infection proceeded, and covered most of the cell surface by 16 h. Six to 24 h after the infection, the labelling density for each glycoprotein on virus particles remained constant. The labelling density for gD was always higher than that for gC. The patch-like distribution of gold-labelling against gD was often detected on infected cell monolayers at the exponential and late stage of one cycle of virus growth. The labelling density for gD on virus particles was the highest on these produced in Vero and L-929 cells, moderate in MRC-5, BHK-21 and FL cells, and the lowest in HEp-2 cells.",
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T2 - Study of herpes simplex virus glycoproteins on the surface of virus particles and infected cells

AU - Padilla, Jorge A.

AU - Uno, Fumio

AU - Yamada, Masao

AU - Nanba, Hikaru

AU - Nii, Shiro

PY - 1997

Y1 - 1997

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