High-performance liquid chromatographic determination of taurine and hypotaurine using 3,5-dinitrobenzoyl chloride as derivatizing reagent

Noriyoshi Masuoka, Kenzaburoh Yao, Masahiro Kinuta, Jun Ohta, Masahiro Wakimoto, Toshihiko Ubuka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A method for the determination of taurine and hypotaurine in biological samples involving the preparation of their 3,5-dinitrobenzoyl derivatives followed by HPLC was established. Taurine and hypotaurine in aqueous media were reacted with 3,5-dinitrobenzoyl chloride in the presence of triethylamine to prepare 3,5-dinitrobenzoyl derivatives. These derivatives were separated on a C18 reversed-phase column and detected by recording the absorbance at 254 nm. Derivatives of taurine and hypotaurine were obtained in yields of 91.4 ± 3.3 and 85.6 ± 2.6%, respectively. The calibration graphs for taurine and hypotaurine were linear between 2.5 and 500 μM with correlation coefficients of 0.999. The method was applied to the determination of taurine and hypotaurine in human and rat urine and blood and in rat liver and heart.

Original languageEnglish
Pages (from-to)31-35
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume660
Issue number1
DOIs
Publication statusPublished - Oct 3 1994

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Taurine
Liquids
Derivatives
Rats
Liver
Blood
hypotaurine
3,5-dinitrobenzoyl chloride
Calibration

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

High-performance liquid chromatographic determination of taurine and hypotaurine using 3,5-dinitrobenzoyl chloride as derivatizing reagent. / Masuoka, Noriyoshi; Yao, Kenzaburoh; Kinuta, Masahiro; Ohta, Jun; Wakimoto, Masahiro; Ubuka, Toshihiko.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 660, No. 1, 03.10.1994, p. 31-35.

Research output: Contribution to journalArticle

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