High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPß, on AZ-521

Takahiro Kimura, Akihiro Wada, Masaaki Nakayama, Ken ichi Ogushi, Yoshito Nishi, Blanquita B. De Guzman, Joel Moss, Toshiya Hirayama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

VacA, a secretory product of Helicobacter pylori, binds to its cell surface receptor, receptor tyrosine phosphatase (RPTP) β, leading to cytoplasmic vacuolization of gastric epithelial AZ-521 cells. VacA binding to the cell surface and VacA-dependent vacuolization were inhibited by cell culture media containing fetal calf serum (FCS). The high molecular weight fraction of FCS isolated by Superose 12 gel filtration chromatography inhibited VacA binding, whereas only weak effects were observed with other fractions. These data show that the high molecular weight fraction of FCS inhibits VacA action though its ability to block toxin binding to its receptor, RPTPβ, on AZ-521 cells.

Original languageEnglish
Pages (from-to)105-107
Number of pages3
JournalMicrobiology and Immunology
Volume47
Issue number1
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

Phosphoric Monoester Hydrolases
Helicobacter pylori
Molecular Weight
Serum
Cell Surface Receptors
Gel Chromatography
Culture Media
Stomach
Cell Culture Techniques
tyrosine receptor

Keywords

  • Helicobacter pylori
  • RPTPβ
  • Toxin receptor
  • VacA
  • Vacuolating cytotoxin

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Kimura, T., Wada, A., Nakayama, M., Ogushi, K. I., Nishi, Y., De Guzman, B. B., ... Hirayama, T. (2003). High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPß, on AZ-521. Microbiology and Immunology, 47(1), 105-107.

High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPß, on AZ-521. / Kimura, Takahiro; Wada, Akihiro; Nakayama, Masaaki; Ogushi, Ken ichi; Nishi, Yoshito; De Guzman, Blanquita B.; Moss, Joel; Hirayama, Toshiya.

In: Microbiology and Immunology, Vol. 47, No. 1, 2003, p. 105-107.

Research output: Contribution to journalArticle

Kimura, T, Wada, A, Nakayama, M, Ogushi, KI, Nishi, Y, De Guzman, BB, Moss, J & Hirayama, T 2003, 'High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPß, on AZ-521', Microbiology and Immunology, vol. 47, no. 1, pp. 105-107.
Kimura, Takahiro ; Wada, Akihiro ; Nakayama, Masaaki ; Ogushi, Ken ichi ; Nishi, Yoshito ; De Guzman, Blanquita B. ; Moss, Joel ; Hirayama, Toshiya. / High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPß, on AZ-521. In: Microbiology and Immunology. 2003 ; Vol. 47, No. 1. pp. 105-107.
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