High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPß, on AZ-521

Takahiro Kimura; Akihiro Wada; Masaaki Nakayama; Ken ichi Ogushi; Yoshito Nishi; Blanquita B. De Guzman; Joel Moss; Toshiya Hirayama

doi:10.1111/j.1348-0421.2003.tb02792.x

High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPß, on AZ-521

Takahiro Kimura, Akihiro Wada, Masaaki Nakayama, Ken ichi Ogushi, Yoshito Nishi, Blanquita B. De Guzman, Joel Moss, Toshiya Hirayama

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

VacA, a secretory product of Helicobacter pylori, binds to its cell surface receptor, receptor tyrosine phosphatase (RPTP) β, leading to cytoplasmic vacuolization of gastric epithelial AZ-521 cells. VacA binding to the cell surface and VacA-dependent vacuolization were inhibited by cell culture media containing fetal calf serum (FCS). The high molecular weight fraction of FCS isolated by Superose 12 gel filtration chromatography inhibited VacA binding, whereas only weak effects were observed with other fractions. These data show that the high molecular weight fraction of FCS inhibits VacA action though its ability to block toxin binding to its receptor, RPTPβ, on AZ-521 cells.

Original language	English
Pages (from-to)	105-107
Number of pages	3
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	47
Issue number	1
DOIs	https://doi.org/10.1111/j.1348-0421.2003.tb02792.x
Publication status	Published - 2003
Externally published	Yes

Keywords

Helicobacter pylori
RPTPβ
Toxin receptor
VacA
Vacuolating cytotoxin

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPß, on AZ-521. / Kimura, Takahiro; Wada, Akihiro; Nakayama, Masaaki; Ogushi, Ken ichi; Nishi, Yoshito; De Guzman, Blanquita B.; Moss, Joel; Hirayama, Toshiya.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 47, No. 1, 2003, p. 105-107.

Research output: Contribution to journal › Article › peer-review

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title = "High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTP{\ss}, on AZ-521",

abstract = "VacA, a secretory product of Helicobacter pylori, binds to its cell surface receptor, receptor tyrosine phosphatase (RPTP) β, leading to cytoplasmic vacuolization of gastric epithelial AZ-521 cells. VacA binding to the cell surface and VacA-dependent vacuolization were inhibited by cell culture media containing fetal calf serum (FCS). The high molecular weight fraction of FCS isolated by Superose 12 gel filtration chromatography inhibited VacA binding, whereas only weak effects were observed with other fractions. These data show that the high molecular weight fraction of FCS inhibits VacA action though its ability to block toxin binding to its receptor, RPTPβ, on AZ-521 cells.",

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author = "Takahiro Kimura and Akihiro Wada and Masaaki Nakayama and Ogushi, {Ken ichi} and Yoshito Nishi and {De Guzman}, {Blanquita B.} and Joel Moss and Toshiya Hirayama",

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AU - Kimura, Takahiro

AU - Wada, Akihiro

AU - Nakayama, Masaaki

AU - Ogushi, Ken ichi

AU - Nishi, Yoshito

AU - De Guzman, Blanquita B.

AU - Moss, Joel

AU - Hirayama, Toshiya

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AB - VacA, a secretory product of Helicobacter pylori, binds to its cell surface receptor, receptor tyrosine phosphatase (RPTP) β, leading to cytoplasmic vacuolization of gastric epithelial AZ-521 cells. VacA binding to the cell surface and VacA-dependent vacuolization were inhibited by cell culture media containing fetal calf serum (FCS). The high molecular weight fraction of FCS isolated by Superose 12 gel filtration chromatography inhibited VacA binding, whereas only weak effects were observed with other fractions. These data show that the high molecular weight fraction of FCS inhibits VacA action though its ability to block toxin binding to its receptor, RPTPβ, on AZ-521 cells.

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