TY - JOUR
T1 - High-level expression of biologically active chicken prolactin in E. coli
AU - Ohkubo, Takeshi
AU - Tanaka, Minuro
AU - Nakashima, Kunio
AU - Shimada, Kiyoshi
AU - Saito, Noboru
AU - Sato, Koji
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1993/5
Y1 - 1993/5
N2 - 1. 1. A large quantity of chicken prolactin (cPRL) was produced by manipulating the cPRL cDNA clone and an expression vector pKK223-3. To augment the production of the hormonal protein in E. coli, in addition to the potent tac promoter, a unique DNA linker containing a pair of Shine-Dalgarno sequences and a short preceding cistron sequence was inserted into adjacent 5'-region of the coding region. 2. 2. In sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis, recombinant cPRL protein exhibited a molecular mass of 23 kDa. 3. 3. The recombinant cPRL showed equivalent binding kinetics to an antiserum raised against turkey PRL. Also, this product increased the weight of pigeon crop sac mucosa to a degree comparable to that induced by turkey PRL. 4. 4. These results indicate that this recombinant cPRL has immunological and biological activities identical to those of authentic avian PRL.
AB - 1. 1. A large quantity of chicken prolactin (cPRL) was produced by manipulating the cPRL cDNA clone and an expression vector pKK223-3. To augment the production of the hormonal protein in E. coli, in addition to the potent tac promoter, a unique DNA linker containing a pair of Shine-Dalgarno sequences and a short preceding cistron sequence was inserted into adjacent 5'-region of the coding region. 2. 2. In sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis, recombinant cPRL protein exhibited a molecular mass of 23 kDa. 3. 3. The recombinant cPRL showed equivalent binding kinetics to an antiserum raised against turkey PRL. Also, this product increased the weight of pigeon crop sac mucosa to a degree comparable to that induced by turkey PRL. 4. 4. These results indicate that this recombinant cPRL has immunological and biological activities identical to those of authentic avian PRL.
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U2 - 10.1016/0300-9629(93)90183-5
DO - 10.1016/0300-9629(93)90183-5
M3 - Article
C2 - 8099867
AN - SCOPUS:0027162783
VL - 105
SP - 123
EP - 128
JO - Comparative Biochemistry and Physiology -- Part A: Physiology
JF - Comparative Biochemistry and Physiology -- Part A: Physiology
SN - 0300-9629
IS - 1
ER -