High incidence of antinuclear antibodies that recognize the matrix attachment region

Hiroko Tohge, Kimiko Tsutsui, Kuniaki Sano, Sevim Isik, Ken Tsutsui

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The matrix attachment region (MAR) is a distinctive genomic DNA involved in a variety of nuclear processes through association with the nuclear matrix. Recent studies suggest that nuclear matrix is altered in the process of apoptosis and presented to the immune system, leading to the production of autoantibodies against its protein components. To see whether MARs are also recognized by autoantibodies, a collection of human sera containing antinuclear antibodies was screened for the presence of binding activities against cloned MARs. We found that MAR-binding activities are quite common in these sera. There was a positive correlation among the MAR-binding titers for three different MAR probes. As expected, the MAR-binding activity was copurified with serum IgG, and subclass analysis with affinity-purified IgG on MAR-Sepharose showed a predominance of IgG2 isotype. Several lines of evidence implied that the anti-MAR antibodies detected here is distinct from the ordinary anti-DNA antibodies that are reactive to bulk DNA.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume285
Issue number1
DOIs
Publication statusPublished - 2001

Keywords

  • Anti-DNA antibodies
  • Antinuclear antibodies
  • Apoptosis
  • Autoantibodies
  • Autoimmune disease
  • Matrix attachment region
  • Nuclear matrix

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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