High glucose suppresses cathepsin activity in periodontal-ligament-derived fibroblastic cells

F. Nishimura, K. Naruishi, H. Yamada, Takayuki Kono, Shogo Takashiba, Y. Murayama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The accumulation of extracellular matrices and integrins by high glucose has been reported in relation to diabetic complications. We previously reported that PDL cells expressed a higher amount of VLA-5 when cultured in high-glucose (4500 mg/L) medium than those cultured in low-glucose (1100 mg/L) medium. In this study, we aimed to address (1) whether this effect was mediated by the transcriptional level of the gene or the degradative level of the protein, and (2) whether this effect was mediated by TGF-β. The results indicated that the level of mRNA encoding α5 integrin did not change in PDL cells regardless of the concentration of glucose. Alternatively, high glucose suppressed cathepsin B+L activity. Additionally, the level of mRNA encoding TGF-β was not affected by high glucose, nor did an anti-TGF-β neutralizing antibody have an effect on the expression of β5 gene or cathepsin activity. Therefore, the effects of high glucose appeared to be mediated by impaired protein degradation, but not by autocrine TGF-β.

Original languageEnglish
Pages (from-to)1614-1617
Number of pages4
JournalJournal of Dental Research
Volume79
Issue number8
Publication statusPublished - 2000

Fingerprint

Cathepsins
Periodontal Ligament
Glucose
Integrins
Integrin alpha5beta1
Cathepsin L
Cathepsin B
Messenger RNA
Diabetes Complications
Neutralizing Antibodies
Proteolysis
Extracellular Matrix
Gene Expression
Genes

Keywords

  • Cathepsins
  • Hyperglycemia
  • Lysosomal enzyme
  • TGF-β
  • VLA-5

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

High glucose suppresses cathepsin activity in periodontal-ligament-derived fibroblastic cells. / Nishimura, F.; Naruishi, K.; Yamada, H.; Kono, Takayuki; Takashiba, Shogo; Murayama, Y.

In: Journal of Dental Research, Vol. 79, No. 8, 2000, p. 1614-1617.

Research output: Contribution to journalArticle

Nishimura, F. ; Naruishi, K. ; Yamada, H. ; Kono, Takayuki ; Takashiba, Shogo ; Murayama, Y. / High glucose suppresses cathepsin activity in periodontal-ligament-derived fibroblastic cells. In: Journal of Dental Research. 2000 ; Vol. 79, No. 8. pp. 1614-1617.
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