High glucose increases metallothionein expression in renal proximal tubular epithelial cells

Daisuke Ogawa, Masato Asanuma, Ikuko Miyazaki, Hiromi Tachibana, Jun Wada, Norio Sogawa, Takeshi Sugaya, Shinji Kitamura, Yohei Maeshima, Kenichi Shikata, Hirofumi Makino

Research output: Contribution to journalArticle

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Abstract

Metallothionein (MT) is an intracellular metal-binding, cysteine-rich protein, and is a potent antioxidant that protects cells and tissues from oxidative stress. Although the major isoforms MT-1 and -2 (MT-1/-2) are highly inducible in many tissues, the distribution and role of MT-1/-2 in diabetic nephropathy are poorly understood. In this study, diabetes was induced in adult male rats by streptozotocin, and renal tissues were stained with antibodies for MT-1/-2. MT-1/-2 expression was also evaluated in mProx24 cells, a mouse renal proximal tubular epithelial cell line, stimulated with high glucose medium and pretreated with the antioxidant vitamin E. MT-1/-2 expression was gradually and dramatically increased, mainly in the proximal tubular epithelial cells and to a lesser extent in the podocytes in diabetic rats, but was hardly observed in control rats. MT-1/-2 expression was also increased by high glucose stimulation in mProx24 cells. Because the induction of MT was suppressed by pretreatment with vitamin E, the expression of MT-1/-2 is induced, at least in part, by high glucose-induced oxidative stress. These observations suggest that MT-1/-2 is induced in renal proximal tubular epithelial cells as an antioxidant to protect the kidney from oxidative stress, and may offer a novel therapeutic target against diabetic nephropathy.

Original languageEnglish
Article number534872
JournalExperimental Diabetes Research
Volume2011
DOIs
Publication statusPublished - 2011

Fingerprint

Metallothionein
Epithelial Cells
Kidney
Glucose
Oxidative Stress
Antioxidants
Diabetic Nephropathies
Vitamin E
Cells
Proximal
Podocytes
Tissue Distribution
Streptozocin
Cysteine
Metals
Rat
Cell Line
Antibodies

ASJC Scopus subject areas

  • Medicine(all)
  • Endocrinology, Diabetes and Metabolism
  • Music

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High glucose increases metallothionein expression in renal proximal tubular epithelial cells. / Ogawa, Daisuke; Asanuma, Masato; Miyazaki, Ikuko; Tachibana, Hiromi; Wada, Jun; Sogawa, Norio; Sugaya, Takeshi; Kitamura, Shinji; Maeshima, Yohei; Shikata, Kenichi; Makino, Hirofumi.

In: Experimental Diabetes Research, Vol. 2011, 534872, 2011.

Research output: Contribution to journalArticle

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AU - Ogawa, Daisuke

AU - Asanuma, Masato

AU - Miyazaki, Ikuko

AU - Tachibana, Hiromi

AU - Wada, Jun

AU - Sogawa, Norio

AU - Sugaya, Takeshi

AU - Kitamura, Shinji

AU - Maeshima, Yohei

AU - Shikata, Kenichi

AU - Makino, Hirofumi

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