High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima

H. Hisano, Y. Kimoto, H. Hayakawa, J. Takeichi, T. Domae, R. Hashimoto, J. Abe, S. Asano, A. Kanazawa, Y. Shimamoto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet (Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and β-glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations.

Original languageEnglish
Pages (from-to)910-918
Number of pages9
JournalPlant Cell Reports
Volume22
Issue number12
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

Keywords

  • Agrobacterium tumefaciens
  • Beta vulgaris
  • Regeneration
  • Shoot-base tissue
  • Transformation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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