Abstract
Our objective was to clarify the DNA-binding activity of transcription factor NF-κB as related to cytokine induction in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Synovial membranes were obtained during arthroplasty of the knee from 7 patients with RA and 4 patients with OA. Nuclear extracts obtained from the synovial membranes were examined by electrophoretic mobility shift assay to determine the DNA-binding activity of NF-κB. Markedly high DNA-binding activity of NF-κB was detected in the synovial membranes of RA patients, while virtually no activity was observed in those of OA patients. These results suggest that the high induction of NF-κB in the nuclear extracts may be relatively specific to synovial membranes in RA. NF-κB may regulate the production of cytokines at the site of synovial inflammation.
| Original language | English |
|---|---|
| Pages (from-to) | 827-832 |
| Number of pages | 6 |
| Journal | Biochemistry and Molecular Biology International |
| Volume | 37 |
| Issue number | 5 |
| Publication status | Published - 1995 |
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Keywords
- Electrophoretic mobility shift assay
- NF-κB
- Rheumatoid arthritis
- Synovium
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Molecular Biology
Cite this
High DNA-Binding activity of transcription factor NF-κB in synovial membranes of patients with rheumatoid arthritis. / Asahara, H.; Asanuma, Masato; Ogawa, N.; Nishibayashi, S.; Inoue, H.
In: Biochemistry and Molecular Biology International, Vol. 37, No. 5, 1995, p. 827-832.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High DNA-Binding activity of transcription factor NF-κB in synovial membranes of patients with rheumatoid arthritis
AU - Asahara, H.
AU - Asanuma, Masato
AU - Ogawa, N.
AU - Nishibayashi, S.
AU - Inoue, H.
PY - 1995
Y1 - 1995
N2 - Our objective was to clarify the DNA-binding activity of transcription factor NF-κB as related to cytokine induction in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Synovial membranes were obtained during arthroplasty of the knee from 7 patients with RA and 4 patients with OA. Nuclear extracts obtained from the synovial membranes were examined by electrophoretic mobility shift assay to determine the DNA-binding activity of NF-κB. Markedly high DNA-binding activity of NF-κB was detected in the synovial membranes of RA patients, while virtually no activity was observed in those of OA patients. These results suggest that the high induction of NF-κB in the nuclear extracts may be relatively specific to synovial membranes in RA. NF-κB may regulate the production of cytokines at the site of synovial inflammation.
AB - Our objective was to clarify the DNA-binding activity of transcription factor NF-κB as related to cytokine induction in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Synovial membranes were obtained during arthroplasty of the knee from 7 patients with RA and 4 patients with OA. Nuclear extracts obtained from the synovial membranes were examined by electrophoretic mobility shift assay to determine the DNA-binding activity of NF-κB. Markedly high DNA-binding activity of NF-κB was detected in the synovial membranes of RA patients, while virtually no activity was observed in those of OA patients. These results suggest that the high induction of NF-κB in the nuclear extracts may be relatively specific to synovial membranes in RA. NF-κB may regulate the production of cytokines at the site of synovial inflammation.
KW - Electrophoretic mobility shift assay
KW - NF-κB
KW - Rheumatoid arthritis
KW - Synovium
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UR - http://www.scopus.com/inward/citedby.url?scp=0029552889&partnerID=8YFLogxK
M3 - Article
C2 - 8624487
AN - SCOPUS:0029552889
VL - 37
SP - 827
EP - 832
JO - IUBMB Life
JF - IUBMB Life
SN - 1521-6543
IS - 5
ER -