High DNA-Binding activity of transcription factor NF-κB in synovial membranes of patients with rheumatoid arthritis

H. Asahara, M. Asanuma, N. Ogawa, S. Nishibayashi, H. Inoue

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Our objective was to clarify the DNA-binding activity of transcription factor NF-κB as related to cytokine induction in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Synovial membranes were obtained during arthroplasty of the knee from 7 patients with RA and 4 patients with OA. Nuclear extracts obtained from the synovial membranes were examined by electrophoretic mobility shift assay to determine the DNA-binding activity of NF-κB. Markedly high DNA-binding activity of NF-κB was detected in the synovial membranes of RA patients, while virtually no activity was observed in those of OA patients. These results suggest that the high induction of NF-κB in the nuclear extracts may be relatively specific to synovial membranes in RA. NF-κB may regulate the production of cytokines at the site of synovial inflammation.

Original languageEnglish
Pages (from-to)827-832
Number of pages6
JournalBiochemistry and Molecular Biology International
Volume37
Issue number5
Publication statusPublished - Dec 1 1995

Keywords

  • Electrophoretic mobility shift assay
  • NF-κB
  • Rheumatoid arthritis
  • Synovium

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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