Abstract
Our objective was to clarify the DNA-binding activity of transcription factor NF-κB as related to cytokine induction in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Synovial membranes were obtained during arthroplasty of the knee from 7 patients with RA and 4 patients with OA. Nuclear extracts obtained from the synovial membranes were examined by electrophoretic mobility shift assay to determine the DNA-binding activity of NF-κB. Markedly high DNA-binding activity of NF-κB was detected in the synovial membranes of RA patients, while virtually no activity was observed in those of OA patients. These results suggest that the high induction of NF-κB in the nuclear extracts may be relatively specific to synovial membranes in RA. NF-κB may regulate the production of cytokines at the site of synovial inflammation.
Original language | English |
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Pages (from-to) | 827-832 |
Number of pages | 6 |
Journal | Biochemistry and Molecular Biology International |
Volume | 37 |
Issue number | 5 |
Publication status | Published - Dec 1 1995 |
Keywords
- Electrophoretic mobility shift assay
- NF-κB
- Rheumatoid arthritis
- Synovium
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics