High concentration of β-defensin-2 in oral squamous cell carcinoma

Koichi Sawaki, Nobuyoshi Mizukawa, Tomoichiro Yamaai, Tomohito Yoshimoto, Makoto Nakano, Toshio Sugahara

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We have previously reported the presence of human β-defensin-2 (HBD-2), a peptide with antimicrobial and cytotoxic properties, in oral squamous cell carcinomas. The aim of the present study was to measure the concentration of HBD-2 in abnormal tissues such as oral squamous cell carcinomas. HBD-2 was extracted from tissue samples in the presence of retinoic acid and subjected to reversed-phase HPLC. The fraction representing peak 17 (P 17) was obtained by elution using a linear gradient of acetonitrile. Amino acid sequencing and homology studies were subsequently performed, and the molecular weight of P17 was calculated to be 4327.02 daltons. This molecular weight was consistent with HBD-2. The concentration of HBD-2 in the oral squamous cell carcinoma samples was 3.85±1.87 μg/mg which was much higher than in normal oral epithelium (0.04±0.02 μg/mg).

Original languageEnglish
Pages (from-to)2103-2107
Number of pages5
JournalAnticancer Research
Volume22
Issue number4
Publication statusPublished - 2002

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Defensins
Squamous Cell Carcinoma
Molecular Weight
Protein Sequence Analysis
Tretinoin
Epithelium
High Pressure Liquid Chromatography
Peptides

Keywords

  • Defensin
  • HBD-2
  • Reversed-phase HPLC
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sawaki, K., Mizukawa, N., Yamaai, T., Yoshimoto, T., Nakano, M., & Sugahara, T. (2002). High concentration of β-defensin-2 in oral squamous cell carcinoma. Anticancer Research, 22(4), 2103-2107.

High concentration of β-defensin-2 in oral squamous cell carcinoma. / Sawaki, Koichi; Mizukawa, Nobuyoshi; Yamaai, Tomoichiro; Yoshimoto, Tomohito; Nakano, Makoto; Sugahara, Toshio.

In: Anticancer Research, Vol. 22, No. 4, 2002, p. 2103-2107.

Research output: Contribution to journalArticle

Sawaki, K, Mizukawa, N, Yamaai, T, Yoshimoto, T, Nakano, M & Sugahara, T 2002, 'High concentration of β-defensin-2 in oral squamous cell carcinoma', Anticancer Research, vol. 22, no. 4, pp. 2103-2107.
Sawaki K, Mizukawa N, Yamaai T, Yoshimoto T, Nakano M, Sugahara T. High concentration of β-defensin-2 in oral squamous cell carcinoma. Anticancer Research. 2002;22(4):2103-2107.
Sawaki, Koichi ; Mizukawa, Nobuyoshi ; Yamaai, Tomoichiro ; Yoshimoto, Tomohito ; Nakano, Makoto ; Sugahara, Toshio. / High concentration of β-defensin-2 in oral squamous cell carcinoma. In: Anticancer Research. 2002 ; Vol. 22, No. 4. pp. 2103-2107.
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