High aluminum resistance in buckwheat: II. oxalic acid detoxifies aluminum internally

Jian Feng Ma, Syuntaro Hiradate, Hideaki Matsumoto

Research output: Contribution to journalArticle

220 Citations (Scopus)

Abstract

Buckwheat (Fagopyrum esculentum Moench. cv Jianxi), which shows high Al resistance, accumulates Al in the leaves. The internal detoxification mechanism was studied by purifying and identifying Al complexes in the leaves and roots. About 90% of Al accumulated in the leaves was found in the cell sap, in which the dominant organic acid was oxalic acid. Purification of the Al complex in the cell sap of leaves by molecular-sieve chromatography resulted in a complex with a ratio of Al to oxalic acid of 1:3. A 13C-nuclear magnetic resonance study of the purified cell sap revealed only one signal at a chemical shift 164.4 ppm, which was assigned to the Al-chelated carboxylic group of oxalic acid. A 27Al-nuclear magnetic resonance analysis revealed one major signal at the chemical shift of 16.0 to 17.0 ppm, with a minor signal at the chemical shift of 11.0 to 12 ppm in both the intact roots and their cell sap, which is consistent with the Al-oxalate complexes at 1:3 and 1:2 ratios, respectively. The purified cell sap was not phytotoxic to root elongation in corn (Zea mays). All of these results indicate that Al tolerance in the roots and leaves of buckwheat is achieved by the formation of a nonphytotoxic Al-oxalate (1:3) complex.

Original languageEnglish
Pages (from-to)753-759
Number of pages7
JournalPlant Physiology
Volume117
Issue number3
Publication statusPublished - 1998

Fingerprint

Fagopyrum
Oxalic Acid
oxalic acid
buckwheat
Aluminum
sap
aluminum
Oxalates
leaves
oxalates
Zea mays
cells
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Fagopyrum esculentum
organic acids and salts
Gel Chromatography
root growth
Acids
corn

ASJC Scopus subject areas

  • Plant Science

Cite this

High aluminum resistance in buckwheat : II. oxalic acid detoxifies aluminum internally. / Ma, Jian Feng; Hiradate, Syuntaro; Matsumoto, Hideaki.

In: Plant Physiology, Vol. 117, No. 3, 1998, p. 753-759.

Research output: Contribution to journalArticle

Ma, Jian Feng ; Hiradate, Syuntaro ; Matsumoto, Hideaki. / High aluminum resistance in buckwheat : II. oxalic acid detoxifies aluminum internally. In: Plant Physiology. 1998 ; Vol. 117, No. 3. pp. 753-759.
@article{ceba9a8064784ffd998574be27fbbd9c,
title = "High aluminum resistance in buckwheat: II. oxalic acid detoxifies aluminum internally",
abstract = "Buckwheat (Fagopyrum esculentum Moench. cv Jianxi), which shows high Al resistance, accumulates Al in the leaves. The internal detoxification mechanism was studied by purifying and identifying Al complexes in the leaves and roots. About 90{\%} of Al accumulated in the leaves was found in the cell sap, in which the dominant organic acid was oxalic acid. Purification of the Al complex in the cell sap of leaves by molecular-sieve chromatography resulted in a complex with a ratio of Al to oxalic acid of 1:3. A 13C-nuclear magnetic resonance study of the purified cell sap revealed only one signal at a chemical shift 164.4 ppm, which was assigned to the Al-chelated carboxylic group of oxalic acid. A 27Al-nuclear magnetic resonance analysis revealed one major signal at the chemical shift of 16.0 to 17.0 ppm, with a minor signal at the chemical shift of 11.0 to 12 ppm in both the intact roots and their cell sap, which is consistent with the Al-oxalate complexes at 1:3 and 1:2 ratios, respectively. The purified cell sap was not phytotoxic to root elongation in corn (Zea mays). All of these results indicate that Al tolerance in the roots and leaves of buckwheat is achieved by the formation of a nonphytotoxic Al-oxalate (1:3) complex.",
author = "Ma, {Jian Feng} and Syuntaro Hiradate and Hideaki Matsumoto",
year = "1998",
language = "English",
volume = "117",
pages = "753--759",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "3",

}

TY - JOUR

T1 - High aluminum resistance in buckwheat

T2 - II. oxalic acid detoxifies aluminum internally

AU - Ma, Jian Feng

AU - Hiradate, Syuntaro

AU - Matsumoto, Hideaki

PY - 1998

Y1 - 1998

N2 - Buckwheat (Fagopyrum esculentum Moench. cv Jianxi), which shows high Al resistance, accumulates Al in the leaves. The internal detoxification mechanism was studied by purifying and identifying Al complexes in the leaves and roots. About 90% of Al accumulated in the leaves was found in the cell sap, in which the dominant organic acid was oxalic acid. Purification of the Al complex in the cell sap of leaves by molecular-sieve chromatography resulted in a complex with a ratio of Al to oxalic acid of 1:3. A 13C-nuclear magnetic resonance study of the purified cell sap revealed only one signal at a chemical shift 164.4 ppm, which was assigned to the Al-chelated carboxylic group of oxalic acid. A 27Al-nuclear magnetic resonance analysis revealed one major signal at the chemical shift of 16.0 to 17.0 ppm, with a minor signal at the chemical shift of 11.0 to 12 ppm in both the intact roots and their cell sap, which is consistent with the Al-oxalate complexes at 1:3 and 1:2 ratios, respectively. The purified cell sap was not phytotoxic to root elongation in corn (Zea mays). All of these results indicate that Al tolerance in the roots and leaves of buckwheat is achieved by the formation of a nonphytotoxic Al-oxalate (1:3) complex.

AB - Buckwheat (Fagopyrum esculentum Moench. cv Jianxi), which shows high Al resistance, accumulates Al in the leaves. The internal detoxification mechanism was studied by purifying and identifying Al complexes in the leaves and roots. About 90% of Al accumulated in the leaves was found in the cell sap, in which the dominant organic acid was oxalic acid. Purification of the Al complex in the cell sap of leaves by molecular-sieve chromatography resulted in a complex with a ratio of Al to oxalic acid of 1:3. A 13C-nuclear magnetic resonance study of the purified cell sap revealed only one signal at a chemical shift 164.4 ppm, which was assigned to the Al-chelated carboxylic group of oxalic acid. A 27Al-nuclear magnetic resonance analysis revealed one major signal at the chemical shift of 16.0 to 17.0 ppm, with a minor signal at the chemical shift of 11.0 to 12 ppm in both the intact roots and their cell sap, which is consistent with the Al-oxalate complexes at 1:3 and 1:2 ratios, respectively. The purified cell sap was not phytotoxic to root elongation in corn (Zea mays). All of these results indicate that Al tolerance in the roots and leaves of buckwheat is achieved by the formation of a nonphytotoxic Al-oxalate (1:3) complex.

UR - http://www.scopus.com/inward/record.url?scp=0001185836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001185836&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0001185836

VL - 117

SP - 753

EP - 759

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -