High-Affinity K+ Transporters from a Halophyte, Sporobolus virginicus, Mediate Both K+ and Na+ Transport in Transgenic Arabidopsis, X. laevis Oocytes and Yeast

Yuichi Tada, Chisato Endo, Maki Katsuhara, Tomoaki Horie, Mineo Shibasaka, Yoshiki Nakahara, Takamitsu Kurusu

Research output: Contribution to journalArticle

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Abstract

Class II high-affinity potassium transporters (HKTs) have been proposed to mediate Na+-K+ co-transport in plants, as well as Na+ and K+ homeostasis under K+-starved and saline environments. We identified class II HKTs, namely SvHKT2;1 and SvHKT2;2 (SvHKTs), from the halophytic turf grass, Sporobolus virginicus. SvHKT2;2 expression in S. virginicus was up-regulated by NaCl treatment, while SvHKT2;1 expression was assumed to be up-regulated by K+ starvation and down-regulated by NaCl treatment. Localization analysis revealed SvHKTs predominantly targeted the plasma membrane. SvHKTs complemented K+ uptake deficiency in mutant yeast, and showed both inward and outward K+ and Na+ transport activity in Xenopus laevis oocytes. When constitutively expressed in Arabidopsis, SvHKTs mediated K+ and Na+ accumulation in shoots under K+-starved conditions, and the K+ concentration in xylem saps of transformants was also higher than in those of wild-type plants. These results suggest transporter-enhanced K+ and Na+ uploading to the xylem from xylem parenchyma cells. Together, our data demonstrate that SvHKTs mediate both outward and inward K+ and Na+ transport in X. laevis oocytes, and possibly in plant and yeast cells, depending on the ionic conditions.

Original languageEnglish
Pages (from-to)176-187
Number of pages12
JournalPlant & cell physiology
Volume60
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Sporobolus virginicus
Xylem
Arabidopsis
Oocytes
xylem
transporters
oocytes
Yeasts
Xenopus laevis
genetically modified organisms
yeasts
Plant Cells
turf grasses
Starvation
Poaceae
sap
parenchyma (plant tissue)
starvation
homeostasis
Potassium

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

High-Affinity K+ Transporters from a Halophyte, Sporobolus virginicus, Mediate Both K+ and Na+ Transport in Transgenic Arabidopsis, X. laevis Oocytes and Yeast. / Tada, Yuichi; Endo, Chisato; Katsuhara, Maki; Horie, Tomoaki; Shibasaka, Mineo; Nakahara, Yoshiki; Kurusu, Takamitsu.

In: Plant & cell physiology, Vol. 60, No. 1, 01.01.2019, p. 176-187.

Research output: Contribution to journalArticle

Tada, Yuichi ; Endo, Chisato ; Katsuhara, Maki ; Horie, Tomoaki ; Shibasaka, Mineo ; Nakahara, Yoshiki ; Kurusu, Takamitsu. / High-Affinity K+ Transporters from a Halophyte, Sporobolus virginicus, Mediate Both K+ and Na+ Transport in Transgenic Arabidopsis, X. laevis Oocytes and Yeast. In: Plant & cell physiology. 2019 ; Vol. 60, No. 1. pp. 176-187.
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