Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging

Shinji Miwa, Shuya Yano, Hiroaki Kimura, Mako Yamamoto, Makoto Toneri, Takashi Murakami, Katsuhiro Hayashi, Norio Yamamoto, Toshiyoshi Fujiwara, Hiroyuki Tsuchiya, Robert M. Hoffman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The present study analyzed the heterogeneous cell-cycle dependence and fate of single cancer cells in a population treated with UVB using a fluorescence ubiquitination-based cell-cycle (FUCCI) imaging system. HeLa cells expressing FUCCI were irradiated by 100 or 200 J/m2 UVB. Modulation of the cell-cycle and apoptosis were observed by time-lapse confocal microscopy imaging every 30 min for 72 h. Correlation between cell survival and factors including cell-cycle phase at the time of the irradiation of UVB, mitosis and the G1/S transition were analyzed using the Kaplan–Meier method along with the log rank test. Time-lapse FUCCI imaging of HeLa cells demonstrated that UVB irradiation induced cell-cycle arrest in S/G2/M phase in the majority of the cells. The cells irradiated by 100 or 200 J/m2 UVB during G0/G1 phase had a higher survival rate than the cells irradiated during S/G2/M phase. A minority of cells could escape S/G2/M arrest and undergo mitosis which significantly correlated with decreased survival of the cells. In contrast, G1/S transition significantly correlated with increased survival of the cells after UVB irradiation. UVB at 200 J/m2 resulted in a greater number of apoptotic cells

Original languageEnglish
Pages (from-to)1932-1937
Number of pages6
JournalCell Cycle
Volume14
Issue number12
DOIs
Publication statusPublished - Jan 1 2015

Keywords

  • Apoptosis
  • Cell-cycle
  • FUCCI
  • HeLa
  • Heterogeneity
  • Mitosis
  • Survival analysis
  • Time-lapse imaging
  • UVB

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Miwa, S., Yano, S., Kimura, H., Yamamoto, M., Toneri, M., Murakami, T., Hayashi, K., Yamamoto, N., Fujiwara, T., Tsuchiya, H., & Hoffman, R. M. (2015). Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging. Cell Cycle, 14(12), 1932-1937. https://doi.org/10.1080/15384101.2015.1033598