Heterogeneous behavior of anti-β2-glycoprotein I antibodies on various commercially available enzyme immunoassay plates coated with β2- glycoprotein I

Akito Tsutsumi, Kenji Ichikawa, Eiji Matsuura, Ken ichi Sawada, Takao Koike

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Objective. To evaluate commercially available enzyme immunoassay (EIA) plates for the measurement of anti-β2-glycoprotein I autoantibody (anti- β2-GPI). Methods. Sera from 10 patients with the antiphospholipid syndrome, and 3 monoclonal anti-β2-GPI antibodies established from patients with antiphospholipid syndrome, were assayed for binding to solid phase β2-GPI on 20 commercially available plates. Results. Several commercially available EIA plates were found to be of potential value for the measurement of anti- β2-GPI autoantibody. Some plates were unsuitable for anti-β2-GPI detection, possibly due to less β2-GPI on the plates, or to differences in the nature of conformational changes of β2-GPI induced by the plates. Conclusion. Differences among EIA plates need to be considered when measuring β2-GPI antibodies.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalJournal of Rheumatology
Issue number2
Publication statusPublished - 2000
Externally publishedYes


  • Anti-β-glycoprotein I antibodies
  • Antiphospholipid Syndrome
  • Enzyme Immunoassay

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology


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