Heterogeneous behavior of anti-β2-glycoprotein I antibodies on various commercially available enzyme immunoassay plates coated with β2- glycoprotein I

Akito Tsutsumi, Kenji Ichikawa, Eiji Matsuura, Ken ichi Sawada, Takao Koike

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective. To evaluate commercially available enzyme immunoassay (EIA) plates for the measurement of anti-β2-glycoprotein I autoantibody (anti- β2-GPI). Methods. Sera from 10 patients with the antiphospholipid syndrome, and 3 monoclonal anti-β2-GPI antibodies established from patients with antiphospholipid syndrome, were assayed for binding to solid phase β2-GPI on 20 commercially available plates. Results. Several commercially available EIA plates were found to be of potential value for the measurement of anti- β2-GPI autoantibody. Some plates were unsuitable for anti-β2-GPI detection, possibly due to less β2-GPI on the plates, or to differences in the nature of conformational changes of β2-GPI induced by the plates. Conclusion. Differences among EIA plates need to be considered when measuring β2-GPI antibodies.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalJournal of Rheumatology
Volume27
Issue number2
Publication statusPublished - 2000
Externally publishedYes

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Immunoenzyme Techniques
Autoantibodies
Glycoproteins
Antiphospholipid Syndrome
Antibodies
Serum

Keywords

  • Anti-β-glycoprotein I antibodies
  • Antiphospholipid Syndrome
  • Enzyme Immunoassay

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Heterogeneous behavior of anti-β2-glycoprotein I antibodies on various commercially available enzyme immunoassay plates coated with β2- glycoprotein I. / Tsutsumi, Akito; Ichikawa, Kenji; Matsuura, Eiji; Sawada, Ken ichi; Koike, Takao.

In: Journal of Rheumatology, Vol. 27, No. 2, 2000, p. 391-396.

Research output: Contribution to journalArticle

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