Heterogeneity of anticardiolipin antibodies defined by the anticardiolipin cofactor

Eiji Matsuura, Yoshiko Igarashi, Masao Fujimoto, Kenji Ichikawa, Takahiro Suzuki, Takayuki Sumida, Tatsuji Yasuda, Takao Koike

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Abstract

Anticardiolipin antibodies (aCL) found in sera from patients with SLE react with cardiolipin (CL) in the presence of a 50-kDa serum cofactor. The cofactor, which was identified to be β2-glycoprotein I by sequencing the N-terminal amino acids, not only enhances CL binding by antibodies in SLE but also depresses it by antibodies associated with syphilis. Cofactor-dependent binding of aCL in SLE to solid phase CL was competitively inhibited by the simultaneous addition of fluid phase CL but was unaffected by either prior or simultaneous addition of a high excess of the cofactor. Binding of aCL in syphilis to solid phase CL was competitively inhibited by either addition of the cofactor or fluid phase CL. aCL in SLE reacted with CL, PS, and PA in the presence of cofactor. In contrast, biotinyl-cofactor bound directly to these anionic phospholipids (PL) and also to PG. These results show that the cofactor-CL complex bears an epitope that confers recognition specificity for aCL in SLE, in contrast with direct CL recognition by syphilitic aCL. The direct binding of the cofactor to PL suggests that the cofactor dependence of aCL binding to PL is due to recognition by aCL of a unique epitope generated upon the formation of the cofactor-CL complex.

Original languageEnglish
Pages (from-to)3885-3891
Number of pages7
JournalJournal of Immunology
Volume148
Issue number12
Publication statusPublished - Jun 15 1992

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beta 2-Glycoprotein I
Anticardiolipin Antibodies
Cardiolipins
Phospholipids
Syphilis
Epitopes
Antibodies
Serum
Glycoproteins

ASJC Scopus subject areas

  • Immunology

Cite this

Matsuura, E., Igarashi, Y., Fujimoto, M., Ichikawa, K., Suzuki, T., Sumida, T., ... Koike, T. (1992). Heterogeneity of anticardiolipin antibodies defined by the anticardiolipin cofactor. Journal of Immunology, 148(12), 3885-3891.

Heterogeneity of anticardiolipin antibodies defined by the anticardiolipin cofactor. / Matsuura, Eiji; Igarashi, Yoshiko; Fujimoto, Masao; Ichikawa, Kenji; Suzuki, Takahiro; Sumida, Takayuki; Yasuda, Tatsuji; Koike, Takao.

In: Journal of Immunology, Vol. 148, No. 12, 15.06.1992, p. 3885-3891.

Research output: Contribution to journalArticle

Matsuura, E, Igarashi, Y, Fujimoto, M, Ichikawa, K, Suzuki, T, Sumida, T, Yasuda, T & Koike, T 1992, 'Heterogeneity of anticardiolipin antibodies defined by the anticardiolipin cofactor', Journal of Immunology, vol. 148, no. 12, pp. 3885-3891.
Matsuura E, Igarashi Y, Fujimoto M, Ichikawa K, Suzuki T, Sumida T et al. Heterogeneity of anticardiolipin antibodies defined by the anticardiolipin cofactor. Journal of Immunology. 1992 Jun 15;148(12):3885-3891.
Matsuura, Eiji ; Igarashi, Yoshiko ; Fujimoto, Masao ; Ichikawa, Kenji ; Suzuki, Takahiro ; Sumida, Takayuki ; Yasuda, Tatsuji ; Koike, Takao. / Heterogeneity of anticardiolipin antibodies defined by the anticardiolipin cofactor. In: Journal of Immunology. 1992 ; Vol. 148, No. 12. pp. 3885-3891.
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