Hesperetin glucuronides induce adipocyte differentiation via activation and expression of peroxisome proliferator-activated receptor-γ

Kanae Gamo, Hiroyuki Miyachi, Kayoko Nakamura, Nobuyasu Matsuura

Research output: Contribution to journalArticle

6 Citations (Scopus)


In previous reports, hesperidin, a flavonoid glucoside from citrus fruit, is hydrolyzed to hesperetin, an aglycone of hesperidin, and converted to the hesperetin glucuronides (H7-OG and H3′-OG) in vivo and depresses blood glucose levels. But there are no reports on the activity of hesperetin glucuronides. To determine the activity of hesperetin glucuronides, H7-OG and H3′-OG were synthesized and peroxisome proliferator-activated receptor-γ (PPARγ) agonist activity was observed at 250 μM. These glucuronides accelerated the differentiation of 3T3- L1 cells into adipocytes at 10 μM. Furthermore, H7- OG showed additive effects in reporter gene assays and caused noncompetitive reactions in timeresolved fluorescence resonance energy transfer assays with a thiazolidinedione derivative. Our results indicated that hesperetin glucuronides activated PPARγ, accelerated adipocyte differentiation.

Original languageEnglish
Pages (from-to)1052-1059
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Issue number6
Publication statusPublished - Jan 1 2014



  • Glucuronide
  • Hesperidin
  • PPARγ

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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