Hesperetin glucuronides induce adipocyte differentiation via activation and expression of peroxisome proliferator-activated receptor-γ

Kanae Gamo, Hiroyuki Miyachi, Kayoko Nakamura, Nobuyasu Matsuura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In previous reports, hesperidin, a flavonoid glucoside from citrus fruit, is hydrolyzed to hesperetin, an aglycone of hesperidin, and converted to the hesperetin glucuronides (H7-OG and H3′-OG) in vivo and depresses blood glucose levels. But there are no reports on the activity of hesperetin glucuronides. To determine the activity of hesperetin glucuronides, H7-OG and H3′-OG were synthesized and peroxisome proliferator-activated receptor-γ (PPARγ) agonist activity was observed at 250 μM. These glucuronides accelerated the differentiation of 3T3- L1 cells into adipocytes at 10 μM. Furthermore, H7- OG showed additive effects in reporter gene assays and caused noncompetitive reactions in timeresolved fluorescence resonance energy transfer assays with a thiazolidinedione derivative. Our results indicated that hesperetin glucuronides activated PPARγ, accelerated adipocyte differentiation.

Original languageEnglish
Pages (from-to)1052-1059
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Peroxisome Proliferator-Activated Receptors
Glucuronides
Adipocytes
Chemical activation
Hesperidin
Assays
Citrus fruits
3T3-L1 Cells
Fluorescence Resonance Energy Transfer
Citrus
Glucosides
Reporter Genes
Flavonoids
Blood Glucose
Genes
hesperetin
Derivatives

Keywords

  • Glucuronide
  • Hesperidin
  • PPARγ
  • TR-FRET

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry
  • Medicine(all)

Cite this

Hesperetin glucuronides induce adipocyte differentiation via activation and expression of peroxisome proliferator-activated receptor-γ. / Gamo, Kanae; Miyachi, Hiroyuki; Nakamura, Kayoko; Matsuura, Nobuyasu.

In: Bioscience, Biotechnology and Biochemistry, Vol. 78, No. 6, 2014, p. 1052-1059.

Research output: Contribution to journalArticle

Gamo, Kanae ; Miyachi, Hiroyuki ; Nakamura, Kayoko ; Matsuura, Nobuyasu. / Hesperetin glucuronides induce adipocyte differentiation via activation and expression of peroxisome proliferator-activated receptor-γ. In: Bioscience, Biotechnology and Biochemistry. 2014 ; Vol. 78, No. 6. pp. 1052-1059.
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