Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells

F. Goshima, D. Watanabe, H. Takakuwa, K. Wada, T. Daikoku, Masao Yamada, Y. Nishiyama

Research output: Contribution to journalArticle

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Abstract

A previous study using a mutant lacking the UL17 gene has suggested that the UL17 protein of herpes simplex virus type 1 (HSV-1) is required for the cleavage/packaging of viral DNA. In this study, we have raised a rabbit polyclonal antiserum which specifically reacted with the UL17 protein which has an apparent molecular mass of 78-kDa in the lysates of HSV types 1- and 2-infected Vero cells. Western blot analysis of intracellular capsids demonstrates that the UL17 protein was associated with B and C capsids. Indirect immunofluorescence studies reveal that it colocalized with the major capsid protein VP5 and the scaffoling protein ICP35 within the nucleus. These results suggest that the association of the UL17 protein with immature B-type capsids is important for its role in cleavage/packaging.

Original languageEnglish
Pages (from-to)417-426
Number of pages10
JournalArchives of Virology
Volume145
Issue number2
Publication statusPublished - 2000

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Capsid
Simplexvirus
Human Herpesvirus 1
Product Packaging
Proteins
Vero Cells
Human Herpesvirus 2
Viral DNA
Capsid Proteins
Indirect Fluorescent Antibody Technique
Immune Sera
Western Blotting
Rabbits
Genes

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Goshima, F., Watanabe, D., Takakuwa, H., Wada, K., Daikoku, T., Yamada, M., & Nishiyama, Y. (2000). Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells. Archives of Virology, 145(2), 417-426.

Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells. / Goshima, F.; Watanabe, D.; Takakuwa, H.; Wada, K.; Daikoku, T.; Yamada, Masao; Nishiyama, Y.

In: Archives of Virology, Vol. 145, No. 2, 2000, p. 417-426.

Research output: Contribution to journalArticle

Goshima, F, Watanabe, D, Takakuwa, H, Wada, K, Daikoku, T, Yamada, M & Nishiyama, Y 2000, 'Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells', Archives of Virology, vol. 145, no. 2, pp. 417-426.
Goshima, F. ; Watanabe, D. ; Takakuwa, H. ; Wada, K. ; Daikoku, T. ; Yamada, Masao ; Nishiyama, Y. / Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells. In: Archives of Virology. 2000 ; Vol. 145, No. 2. pp. 417-426.
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