Herpes simplex virus type 1 DNA amplified as bacterial artificial chromosome in Escherichia coli

Rescue of replication-competent virus progeny and packaging of amplicon vectors

Yoshinaga Saeki, Tomotsugu Ichikawa, Akane Saeki, E. Antonio Chiocca, Kurt Tobler, Mathias Ackermann, Xandra O. Breakefield, Cornel Fraefel

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

Herpes simplex virus type 1 (HSV-1)-based amplicon vectors contain only ~ 1% of the 152-kb HSV-1 genome, and consequently, replication and packaging into virions depends on helper functions. These helper functions have been provided conventionally by a helper virus, usually a replication-defective mutant of HSV-1, or more recently, by a set of five cosmids that overlap and represent the genome of HSV-1 deleted for DNA cleavage/packaging signals (pac). In the absence of pac signals, potential HSV-1 genomes that are reconstituted from the cosmids via homologous recombination are not packageable. The resulting amplicon stocks are, therefore, virtually free of contaminating helper virus. To simplify this packing system, the HSV-1 genome was cloned and maintained stably as a single-copy, F plasmid-based bacterial artificial chromosome in E. coli. Such a plasmid containing the HSV-1 genome deleted for the pac signals (fHSV Δ pac) did not generate replication-competent progeny virus on transfection into mammalian cells, but rather, it was able to support the packaging of cotransfected amplicon DNA that contained a functional pac signal. The resulting amplicon vector stocks had titers of up to 107 transducing units per milliliter of culture medium and efficiently transduced neural cells in the rat brain, as well as hepatocytes in the rat. The capacity of generating infectious and replication-competent HSV-1 progeny following transfection into mammalian cells was restored after insertion of a pac signal into fHSV Δ pac.

Original languageEnglish
Pages (from-to)2787-2794
Number of pages8
JournalHuman Gene Therapy
Volume9
Issue number18
Publication statusPublished - Dec 10 1998
Externally publishedYes

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Bacterial Artificial Chromosomes
Virus Assembly
Human Herpesvirus 1
Escherichia coli
DNA
Genome
Helper Viruses
Cosmids
Product Packaging
Transfection
Chromosomes, Human, 16-18
DNA Packaging
F Factor
DNA Cleavage
Homologous Recombination
Virion
Culture Media
Hepatocytes
Plasmids
Viruses

ASJC Scopus subject areas

  • Genetics

Cite this

Herpes simplex virus type 1 DNA amplified as bacterial artificial chromosome in Escherichia coli : Rescue of replication-competent virus progeny and packaging of amplicon vectors. / Saeki, Yoshinaga; Ichikawa, Tomotsugu; Saeki, Akane; Chiocca, E. Antonio; Tobler, Kurt; Ackermann, Mathias; Breakefield, Xandra O.; Fraefel, Cornel.

In: Human Gene Therapy, Vol. 9, No. 18, 10.12.1998, p. 2787-2794.

Research output: Contribution to journalArticle

Saeki, Y, Ichikawa, T, Saeki, A, Chiocca, EA, Tobler, K, Ackermann, M, Breakefield, XO & Fraefel, C 1998, 'Herpes simplex virus type 1 DNA amplified as bacterial artificial chromosome in Escherichia coli: Rescue of replication-competent virus progeny and packaging of amplicon vectors', Human Gene Therapy, vol. 9, no. 18, pp. 2787-2794.
Saeki, Yoshinaga ; Ichikawa, Tomotsugu ; Saeki, Akane ; Chiocca, E. Antonio ; Tobler, Kurt ; Ackermann, Mathias ; Breakefield, Xandra O. ; Fraefel, Cornel. / Herpes simplex virus type 1 DNA amplified as bacterial artificial chromosome in Escherichia coli : Rescue of replication-competent virus progeny and packaging of amplicon vectors. In: Human Gene Therapy. 1998 ; Vol. 9, No. 18. pp. 2787-2794.
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