Herbivore-induced and constitutive volatiles are controlled by different oxylipin-dependent mechanisms in rice

Kadis Mujiono, Tilisa Tohi, Islam S. Sobhy, Yuko Hojo, Tomonori Shinya, Ivan Galis

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Despite the importance of volatile organic compounds (VOCs) for plants, control mechanisms for their basal and stress-induced biosynthesis and release remain unclear. We sampled and characterized headspace and internal leaf volatile pools in rice (Oryza sativa), after a simulated herbivory treatment, which triggers an endogenous jasmonate burst. Certain volatiles, such as linalool, were strongly upregulated by simulated herbivory stress. In contrast, other volatiles, such as β-caryophyllene, were constitutively emitted and fluctuated according to time of day. Transcripts of the linalool synthase gene transiently increased 1–3 h after exposure of rice to simulated herbivory, whereas transcripts of caryophyllene synthase peaked independently at dawn. Unexpectedly, although emission and accumulation patterns of rice inducible and constitutive VOCs were substantially different, both groups of volatiles were compromised in jasmonate-deficient hebiba mutants, which lack the allene oxide cyclase (AOC) gene. This suggests that rice employs at least two distinct oxylipin-dependent mechanisms downstream of AOC to control production of constitutive and herbivore-induced volatiles. Levels of the JA precursor, 12-oxo-phytodienoic acid (OPDA), were correlated with constitutive volatile levels suggesting that OPDA or its derivatives could be involved in control of volatile emission in rice.

    Original languageEnglish
    Pages (from-to)2687-2699
    Number of pages13
    JournalPlant Cell and Environment
    Volume44
    Issue number8
    DOIs
    Publication statusPublished - Aug 2021

    Keywords

    • Diurnal regulation
    • herbivory
    • oxylipins
    • plant volatiles
    • rice (Oryza sativa)

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

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