Herbimycin A suppresses NF-κB activation and tyrosine phosphorylation of JAK2 and the subsequent induction of nitric oxide synthase in C6 glioma cells

Tadashi Nishiya, Takashi Uehara, Yasuyuki Nomura

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Herbimycin A, a potent tyrosine kinase inhibitor, suppressed nitric oxide synthase (NOS) induced by lipopolysaccharide (LPS) and interferon-γ (IFN-γ) in C6 glial cells. LPS activated NF-κB, and this effect was inhibited by pretreatment with herbimycin A. In addition, IFN-γ activated the tyrosine protein kinase, JAK2, and tyrosine-phosphorylation by itself was also inhibited by herbimycin A. These results suggest that herbimycin A suppresses iNOS induction by inhibition of both NF-κB activation caused by LPS, and tyrosine-phosphorylation of JAK2 caused by IFN-γ in C6 glioma cells.

Original languageEnglish
Pages (from-to)333-336
Number of pages4
JournalFEBS Letters
Volume371
Issue number3
DOIs
Publication statusPublished - Sep 11 1995
Externally publishedYes

Keywords

  • C6 glioma cell
  • Herbimycin A
  • JAK2
  • NF-κB
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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