Hepatocyte growth factor receptor is a coreceptor for adeno-associated virus type 2 infection

Yuji Kashiwakura, Kenji Tamayose, Kazuhisa Iwabuchi, Yukihiko Hirai, Takashi Shimada, Kunio Matsumoto, Toshikazu Nakamura, Masami Watanabe, Kazuo Oshimi, Hiroyuki Daida

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

After the first attachment of virus to the cell surface through a primary receptor, efficient entry of virus requires the presence of a coreceptor. For adeno-associated virus type 2 (AAV2) infection, heparan sulfate proteoglycan is supposed as the primary receptor, and avβ5 integrin and FGFR1 are reported to act as coreceptors. In this study, we were able to demonstrate that hepatocyte growth factor receptor, c-Met, is also a coreceptor for AAV2 infection. AAV2-mediated transgene analyses revealed that c-Met expression significantly up-regulated transgene expression without increasing AAV2 cell binding. Moreover, a viral overlay assay elucidated the physical interaction between AAV2 and the β subunit of c-Met. These data suggest that c-Met plays the role of coreceptor for AAV2 infection by facilitating AAV2 interraalization into the cytoplasm.

Original languageEnglish
Pages (from-to)609-614
Number of pages6
JournalJournal of Virology
Volume79
Issue number1
DOIs
Publication statusPublished - Jan 2005

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Kashiwakura, Y., Tamayose, K., Iwabuchi, K., Hirai, Y., Shimada, T., Matsumoto, K., Nakamura, T., Watanabe, M., Oshimi, K., & Daida, H. (2005). Hepatocyte growth factor receptor is a coreceptor for adeno-associated virus type 2 infection. Journal of Virology, 79(1), 609-614. https://doi.org/10.1128/JVI.79.1.609-614.2005