Hepatocyte growth factor is a regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum

Chikako Fujiwara, Shuko Murakami, Hiroaki Taniguchi, Yoko Miyamoto, Ryosuke Sakumoto, Sakae Takeuchi, Sumio Takahashi, Kiyoshi Okuda

Research output: Contribution to journalArticle

Abstract

Active angiogenesis occurs during early luteal development. Angiogenesis in the corpus luteum (CL) is regulated by various growth factors in many species. The purpose of the present study was to determine the effect of hepatocyte growth factor (HGF) on the proliferation of the microvascular endothelial cells derived from developing bovine CL. The expression of HGF and HGF receptor (c-met) mRNAs in cultured bovine endothelial cells was also observed by reverse transcription-polymerase chain reaction analysis. The cells were exposed to HGF (50 ng/ml) for 1, 2, 4, and 6 days. HGF significantly increased the total DNA in endothelial cells at all exposure times (P

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalJournal of Reproduction and Development
Volume48
Issue number1
Publication statusPublished - 2002

Fingerprint

hepatocyte growth factor
corpus luteum
endothelial cells
cattle
angiogenesis
growth factors
exposure duration
reverse transcriptase polymerase chain reaction
DNA
cells

Keywords

  • Corpus luteum
  • Endothelial cell
  • HGF

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Hepatocyte growth factor is a regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum. / Fujiwara, Chikako; Murakami, Shuko; Taniguchi, Hiroaki; Miyamoto, Yoko; Sakumoto, Ryosuke; Takeuchi, Sakae; Takahashi, Sumio; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 48, No. 1, 2002, p. 49-55.

Research output: Contribution to journalArticle

Fujiwara, C, Murakami, S, Taniguchi, H, Miyamoto, Y, Sakumoto, R, Takeuchi, S, Takahashi, S & Okuda, K 2002, 'Hepatocyte growth factor is a regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum', Journal of Reproduction and Development, vol. 48, no. 1, pp. 49-55.
Fujiwara, Chikako ; Murakami, Shuko ; Taniguchi, Hiroaki ; Miyamoto, Yoko ; Sakumoto, Ryosuke ; Takeuchi, Sakae ; Takahashi, Sumio ; Okuda, Kiyoshi. / Hepatocyte growth factor is a regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum. In: Journal of Reproduction and Development. 2002 ; Vol. 48, No. 1. pp. 49-55.
@article{847ce977d2cf4fa1899c44210c77e1cb,
title = "Hepatocyte growth factor is a regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum",
abstract = "Active angiogenesis occurs during early luteal development. Angiogenesis in the corpus luteum (CL) is regulated by various growth factors in many species. The purpose of the present study was to determine the effect of hepatocyte growth factor (HGF) on the proliferation of the microvascular endothelial cells derived from developing bovine CL. The expression of HGF and HGF receptor (c-met) mRNAs in cultured bovine endothelial cells was also observed by reverse transcription-polymerase chain reaction analysis. The cells were exposed to HGF (50 ng/ml) for 1, 2, 4, and 6 days. HGF significantly increased the total DNA in endothelial cells at all exposure times (P",
keywords = "Corpus luteum, Endothelial cell, HGF",
author = "Chikako Fujiwara and Shuko Murakami and Hiroaki Taniguchi and Yoko Miyamoto and Ryosuke Sakumoto and Sakae Takeuchi and Sumio Takahashi and Kiyoshi Okuda",
year = "2002",
language = "English",
volume = "48",
pages = "49--55",
journal = "Journal of Reproduction and Development",
issn = "0916-8818",
publisher = "Japanese Society of Animal Reproduction (JSAR)",
number = "1",

}

TY - JOUR

T1 - Hepatocyte growth factor is a regulator in the proliferation of microvascular endothelial cells in bovine corpus luteum

AU - Fujiwara, Chikako

AU - Murakami, Shuko

AU - Taniguchi, Hiroaki

AU - Miyamoto, Yoko

AU - Sakumoto, Ryosuke

AU - Takeuchi, Sakae

AU - Takahashi, Sumio

AU - Okuda, Kiyoshi

PY - 2002

Y1 - 2002

N2 - Active angiogenesis occurs during early luteal development. Angiogenesis in the corpus luteum (CL) is regulated by various growth factors in many species. The purpose of the present study was to determine the effect of hepatocyte growth factor (HGF) on the proliferation of the microvascular endothelial cells derived from developing bovine CL. The expression of HGF and HGF receptor (c-met) mRNAs in cultured bovine endothelial cells was also observed by reverse transcription-polymerase chain reaction analysis. The cells were exposed to HGF (50 ng/ml) for 1, 2, 4, and 6 days. HGF significantly increased the total DNA in endothelial cells at all exposure times (P

AB - Active angiogenesis occurs during early luteal development. Angiogenesis in the corpus luteum (CL) is regulated by various growth factors in many species. The purpose of the present study was to determine the effect of hepatocyte growth factor (HGF) on the proliferation of the microvascular endothelial cells derived from developing bovine CL. The expression of HGF and HGF receptor (c-met) mRNAs in cultured bovine endothelial cells was also observed by reverse transcription-polymerase chain reaction analysis. The cells were exposed to HGF (50 ng/ml) for 1, 2, 4, and 6 days. HGF significantly increased the total DNA in endothelial cells at all exposure times (P

KW - Corpus luteum

KW - Endothelial cell

KW - HGF

UR - http://www.scopus.com/inward/record.url?scp=53949108996&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53949108996&partnerID=8YFLogxK

M3 - Article

VL - 48

SP - 49

EP - 55

JO - Journal of Reproduction and Development

JF - Journal of Reproduction and Development

SN - 0916-8818

IS - 1

ER -