Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro

S. H. Oh, M. Miyazaki, H. Kouchi, Y. Inoue, Masakiyo Sakaguchi, T. Tsuji, N. Shima, K. Higashio, M. Namba

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Bone marrow (BM) cells originally include alphafetoprotein (AFP)- and c-Met [a receptor for hepatocyte growth factor (HGF)]-expressing cells. In vitro treatment of BM cells with HGF induced albumin-expressing hepatocyte-like cells. Furthermore, those hepatocyte-like cells expressed cytokeratins 8 and 18, which are typically expressed in normal adult hepatocymes. These findings demonstrate that BM cells include AFP-expressing hepatic progenitor cells that can be differentiated into hepatocytes by HGF in culture, indicating that such cultures are useful resources for cell transplantation therapy for liver diseases.

Original languageEnglish
Pages (from-to)500-504
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume279
Issue number2
DOIs
Publication statusPublished - Dec 20 2000

Fingerprint

Hepatocyte Growth Factor
Bone Marrow Cells
Rats
Hepatocytes
Bone
Cells
Proto-Oncogene Proteins c-met
Keratin-8
Keratin-18
Cell Transplantation
Cell- and Tissue-Based Therapy
Liver
Liver Diseases
Albumins
Stem Cells
In Vitro Techniques
Therapeutics

Keywords

  • Bone marrow cells
  • Differentiation
  • Hepatocyte growth factor
  • Hepatocyte lineage

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro. / Oh, S. H.; Miyazaki, M.; Kouchi, H.; Inoue, Y.; Sakaguchi, Masakiyo; Tsuji, T.; Shima, N.; Higashio, K.; Namba, M.

In: Biochemical and Biophysical Research Communications, Vol. 279, No. 2, 20.12.2000, p. 500-504.

Research output: Contribution to journalArticle

Oh, S. H. ; Miyazaki, M. ; Kouchi, H. ; Inoue, Y. ; Sakaguchi, Masakiyo ; Tsuji, T. ; Shima, N. ; Higashio, K. ; Namba, M. / Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 279, No. 2. pp. 500-504.
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