Hepatitis G virus replication in human cultured cells displaying susceptibility to hepatitis C virus infection

Masanori Ikeda, Kazuo Sugiyama, Tetsuya Mizutani, Torahiko Tanaka, Katsuaki Tanaka, Kunitada Shimotohno, Nobuyuki Kato

Research output: Contribution to journalArticle

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Abstract

We recently developed a hepatitis C virus (HCV) replication system using two cultured human cell lines: MT-2C, a human T-cell leukemia virus type I-infected cloned T cell line, and PH5CH, a non-neoplastic human hepatocyte line. In the course of the study, we found evidence that replication of hepatitis G virus (HGV), which has recently been identified, was supported in both MT-2C and PH5CH cells. When these cells were inoculated with serum 1B-3 containing both HCV and HGV obtained from a blood donor, the 5'-noncoding (5'-NC) regions of HCV RNA and HGV RNA were detected by RT-nested PCR in both cell lines more than 30 days postinoculation, and the 3'-noncoding region of HGV RNA was also detected in the both cell lines more than 30 days postinoculation. Sequence analysis of the 5'-NC region of HGV RNA revealed the quasispecies nature of HGV, although the 5'-NC region was highly conserved among HGV isolates. This HGV-infected culture system will be useful for various biological and virological studies of HGV.

Original languageEnglish
Pages (from-to)505-508
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume235
Issue number3
DOIs
Publication statusPublished - Jun 27 1997
Externally publishedYes

Fingerprint

GB virus C
Virus Diseases
Virus Replication
Viruses
Hepacivirus
Cultured Cells
Cells
RNA
Cell Line
T-cells
Human T-lymphotropic virus 1
Blood Donors
Sequence Analysis
Hepatocytes
T-Lymphocytes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hepatitis G virus replication in human cultured cells displaying susceptibility to hepatitis C virus infection. / Ikeda, Masanori; Sugiyama, Kazuo; Mizutani, Tetsuya; Tanaka, Torahiko; Tanaka, Katsuaki; Shimotohno, Kunitada; Kato, Nobuyuki.

In: Biochemical and Biophysical Research Communications, Vol. 235, No. 3, 27.06.1997, p. 505-508.

Research output: Contribution to journalArticle

Ikeda, Masanori ; Sugiyama, Kazuo ; Mizutani, Tetsuya ; Tanaka, Torahiko ; Tanaka, Katsuaki ; Shimotohno, Kunitada ; Kato, Nobuyuki. / Hepatitis G virus replication in human cultured cells displaying susceptibility to hepatitis C virus infection. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 235, No. 3. pp. 505-508.
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