Abstract
We recently developed a hepatitis C virus (HCV) replication system using two cultured human cell lines: MT-2C, a human T-cell leukemia virus type I-infected cloned T cell line, and PH5CH, a non-neoplastic human hepatocyte line. In the course of the study, we found evidence that replication of hepatitis G virus (HGV), which has recently been identified, was supported in both MT-2C and PH5CH cells. When these cells were inoculated with serum 1B-3 containing both HCV and HGV obtained from a blood donor, the 5'-noncoding (5'-NC) regions of HCV RNA and HGV RNA were detected by RT-nested PCR in both cell lines more than 30 days postinoculation, and the 3'-noncoding region of HGV RNA was also detected in the both cell lines more than 30 days postinoculation. Sequence analysis of the 5'-NC region of HGV RNA revealed the quasispecies nature of HGV, although the 5'-NC region was highly conserved among HGV isolates. This HGV-infected culture system will be useful for various biological and virological studies of HGV.
| Original language | English |
|---|---|
| Pages (from-to) | 505-508 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 235 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 27 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Hepatitis G virus replication in human cultured cells displaying susceptibility to hepatitis C virus infection. / Ikeda, Masanori; Sugiyama, Kazuo; Mizutani, Tetsuya; Tanaka, Torahiko; Tanaka, Katsuaki; Shimotohno, Kunitada; Kato, Nobuyuki.
In: Biochemical and Biophysical Research Communications, Vol. 235, No. 3, 27.06.1997, p. 505-508.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hepatitis G virus replication in human cultured cells displaying susceptibility to hepatitis C virus infection
AU - Ikeda, Masanori
AU - Sugiyama, Kazuo
AU - Mizutani, Tetsuya
AU - Tanaka, Torahiko
AU - Tanaka, Katsuaki
AU - Shimotohno, Kunitada
AU - Kato, Nobuyuki
PY - 1997/6/27
Y1 - 1997/6/27
N2 - We recently developed a hepatitis C virus (HCV) replication system using two cultured human cell lines: MT-2C, a human T-cell leukemia virus type I-infected cloned T cell line, and PH5CH, a non-neoplastic human hepatocyte line. In the course of the study, we found evidence that replication of hepatitis G virus (HGV), which has recently been identified, was supported in both MT-2C and PH5CH cells. When these cells were inoculated with serum 1B-3 containing both HCV and HGV obtained from a blood donor, the 5'-noncoding (5'-NC) regions of HCV RNA and HGV RNA were detected by RT-nested PCR in both cell lines more than 30 days postinoculation, and the 3'-noncoding region of HGV RNA was also detected in the both cell lines more than 30 days postinoculation. Sequence analysis of the 5'-NC region of HGV RNA revealed the quasispecies nature of HGV, although the 5'-NC region was highly conserved among HGV isolates. This HGV-infected culture system will be useful for various biological and virological studies of HGV.
AB - We recently developed a hepatitis C virus (HCV) replication system using two cultured human cell lines: MT-2C, a human T-cell leukemia virus type I-infected cloned T cell line, and PH5CH, a non-neoplastic human hepatocyte line. In the course of the study, we found evidence that replication of hepatitis G virus (HGV), which has recently been identified, was supported in both MT-2C and PH5CH cells. When these cells were inoculated with serum 1B-3 containing both HCV and HGV obtained from a blood donor, the 5'-noncoding (5'-NC) regions of HCV RNA and HGV RNA were detected by RT-nested PCR in both cell lines more than 30 days postinoculation, and the 3'-noncoding region of HGV RNA was also detected in the both cell lines more than 30 days postinoculation. Sequence analysis of the 5'-NC region of HGV RNA revealed the quasispecies nature of HGV, although the 5'-NC region was highly conserved among HGV isolates. This HGV-infected culture system will be useful for various biological and virological studies of HGV.
UR - http://www.scopus.com/inward/record.url?scp=0031587958&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031587958&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1997.6818
DO - 10.1006/bbrc.1997.6818
M3 - Article
C2 - 9207185
AN - SCOPUS:0031587958
VL - 235
SP - 505
EP - 508
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -