Hepatitis C virus population dynamics in human lymphocytes and hepatocytes infected in vitro

Nobuyuki Kato, Masanori Ikeda, Kazuo Sugiyama, Tetsuya Mizutani, Torahiko Tanaka, Kunitada Shimotohno

Research output: Contribution to journalArticle

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Abstract

We previously found two cell lines (MT-2 and PH5CH) that were susceptible to hepatitis C virus (HCV) infection. Analysis of the infectivity of sera from HCV-positive blood donors for MT-2 and PH5CH cells suggested the cell tropism of HCV. To investigate further the cell tropism of HCV, the dynamics of HCV populations during culture were examined using three MT-2 clones and three PH5CH clones, infected with inoculum 1B-2. To type HCV populations in these infected cells, the HCV hypervariable region 1 (HVR1) in these cloned cells was characterized by sequence analysis and HpaII digestion analysis, which could distinguish three major HVR1 types (I, II and III) derived from the inoculum 1B-2. It was found that genomes containing HVR1 type I became predominant in MT-2 clones, and genomes containing HVR1 type II became predominant in PH5CH clones during culture after inoculation. These results suggest that inoculum 1B-2 contains both lymphotropic and hepatotropic HCV species, which can be distinguished by HVR1 type. To search for cell type-specific sequences in regions other than HVR1, three HCV cDNA clones (3.4 kb of the 5' noncoding region to the nonstructural 2 region) containing HVR1 type I obtained from HCV-infected MT-2C cells, and three HCV cDNA clones containing HVR1 type II obtained from HCV-infected PH5CH7 cells were sequenced. Following a comparison of the sequences, 11 amino acids were identified as candidates for determinants of the cell tropism of HCV.

Original languageEnglish
Pages (from-to)1859-1869
Number of pages11
JournalJournal of General Virology
Volume79
Issue number8
Publication statusPublished - Aug 1998
Externally publishedYes

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Population Dynamics
Hepacivirus
Hepatocytes
Lymphocytes
Clone Cells
Tropism
In Vitro Techniques
Complementary DNA
Genome
Virus Diseases
Blood Donors
Population
Sequence Analysis
Digestion
Amino Acid Sequence

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Hepatitis C virus population dynamics in human lymphocytes and hepatocytes infected in vitro. / Kato, Nobuyuki; Ikeda, Masanori; Sugiyama, Kazuo; Mizutani, Tetsuya; Tanaka, Torahiko; Shimotohno, Kunitada.

In: Journal of General Virology, Vol. 79, No. 8, 08.1998, p. 1859-1869.

Research output: Contribution to journalArticle

Kato, N, Ikeda, M, Sugiyama, K, Mizutani, T, Tanaka, T & Shimotohno, K 1998, 'Hepatitis C virus population dynamics in human lymphocytes and hepatocytes infected in vitro', Journal of General Virology, vol. 79, no. 8, pp. 1859-1869.
Kato, Nobuyuki ; Ikeda, Masanori ; Sugiyama, Kazuo ; Mizutani, Tetsuya ; Tanaka, Torahiko ; Shimotohno, Kunitada. / Hepatitis C virus population dynamics in human lymphocytes and hepatocytes infected in vitro. In: Journal of General Virology. 1998 ; Vol. 79, No. 8. pp. 1859-1869.
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