Hepatitis C virus population dynamics in human lymphocytes and hepatocytes infected in vitro

Nobuyuki Kato, Masanori Ikeda, Kazuo Sugiyama, Tetsuya Mizutani, Torahiko Tanaka, Kunitada Shimotohno

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We previously found two cell lines (MT-2 and PH5CH) that were susceptible to hepatitis C virus (HCV) infection. Analysis of the infectivity of sera from HCV-positive blood donors for MT-2 and PH5CH cells suggested the cell tropism of HCV. To investigate further the cell tropism of HCV, the dynamics of HCV populations during culture were examined using three MT-2 clones and three PH5CH clones, infected with inoculum 1B-2. To type HCV populations in these infected cells, the HCV hypervariable region 1 (HVR1) in these cloned cells was characterized by sequence analysis and HpaII digestion analysis, which could distinguish three major HVR1 types (I, II and III) derived from the inoculum 1B-2. It was found that genomes containing HVR1 type I became predominant in MT-2 clones, and genomes containing HVR1 type II became predominant in PH5CH clones during culture after inoculation. These results suggest that inoculum 1B-2 contains both lymphotropic and hepatotropic HCV species, which can be distinguished by HVR1 type. To search for cell type-specific sequences in regions other than HVR1, three HCV cDNA clones (3.4 kb of the 5' noncoding region to the nonstructural 2 region) containing HVR1 type I obtained from HCV-infected MT-2C cells, and three HCV cDNA clones containing HVR1 type II obtained from HCV-infected PH5CH7 cells were sequenced. Following a comparison of the sequences, 11 amino acids were identified as candidates for determinants of the cell tropism of HCV.

Original languageEnglish
Pages (from-to)1859-1869
Number of pages11
JournalJournal of General Virology
Volume79
Issue number8
Publication statusPublished - Aug 1998
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Kato, N., Ikeda, M., Sugiyama, K., Mizutani, T., Tanaka, T., & Shimotohno, K. (1998). Hepatitis C virus population dynamics in human lymphocytes and hepatocytes infected in vitro. Journal of General Virology, 79(8), 1859-1869.