Hepatitis C virus genotype distribution in Myanmar: Predominance of genotype 6 and existence of new genotype 6 subtype

Aye Aye Lwin, Toshiyuki Shinji, Myo Khin, Ne Win, Mikako Obika, Shigeru Okada, Norio Koide

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Aim: This study was performed to determine the prevalence and distribution of hepatitis C virus (HCV) genotypes in Myanmar. Methods: A total of 1333 peripheral blood samples were collected from four different border cities of Myanmar. The anti-HCV antibody-positive serum samples were identified. HCV was genotyped by reverse transcriptase polymerase chain reaction, direct DNA sequencing and phylogenetic analysis on the partial core genome. Results: The overall prevalence of HCV infection was 11.6% (154/1333). Regionally, it was 13.5% (47/349) in the north-eastern city, 12.8% (64/501) in the north-western city, 4.2% (16/380) in the southern city and 26.2% (27/103) in the western city. HCV was genotyped in 145/154 (94.2%) samples. Genotype 6 was the most prevalent genotype in this study (71/145, 49%), followed by genotype 3 (57/145, 39.3%), genotype 1 (16/145, 11%), and genotype 2 (1/145, 0.7%). Genotype 6 was mostly found in the northern cities and genotype 3 in the southern and western cities of Myanmar. Multiple HCV genotypes/subtypes were successfully characterized as 1a, 1b, 2a, 3a, 3b, 6m, 6n, and a new 6 subtype. Among them, subtype 6n was the most predominant subtype (38.6%), followed by subtype 3b (29.7%), 3a (9.6%), 6m (9%), 1b (6.9%), 1a (4.1%), new 6 subtype (1.4%) and 2a (0.7%). Subtype 6n was more widely distributed in the northern cities whereas subtype 3b was more common in the western city. The newly discovered genotype 6 subtype was from the northern cities. Conclusions: The results indicate there are regional differences of HCV genotype distribution in Myanmar. There is a distinct geographic variation from other South-East Asian countries in terms of the existence of the new genotype 6 subtype.

Original languageEnglish
Pages (from-to)337-345
Number of pages9
JournalHepatology Research
Volume37
Issue number5
DOIs
Publication statusPublished - May 2007

Fingerprint

Myanmar
Hepacivirus
Genotype
Hepatitis C Antibodies
Virus Diseases
Reverse Transcriptase Polymerase Chain Reaction
DNA Sequence Analysis

Keywords

  • DNA sequencing
  • Genotype
  • HepatitisC virus
  • Myanmar
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Hepatitis C virus genotype distribution in Myanmar : Predominance of genotype 6 and existence of new genotype 6 subtype. / Lwin, Aye Aye; Shinji, Toshiyuki; Khin, Myo; Win, Ne; Obika, Mikako; Okada, Shigeru; Koide, Norio.

In: Hepatology Research, Vol. 37, No. 5, 05.2007, p. 337-345.

Research output: Contribution to journalArticle

Lwin, Aye Aye ; Shinji, Toshiyuki ; Khin, Myo ; Win, Ne ; Obika, Mikako ; Okada, Shigeru ; Koide, Norio. / Hepatitis C virus genotype distribution in Myanmar : Predominance of genotype 6 and existence of new genotype 6 subtype. In: Hepatology Research. 2007 ; Vol. 37, No. 5. pp. 337-345.
@article{4d2cf090323d4aa8b178883ad62e6154,
title = "Hepatitis C virus genotype distribution in Myanmar: Predominance of genotype 6 and existence of new genotype 6 subtype",
abstract = "Aim: This study was performed to determine the prevalence and distribution of hepatitis C virus (HCV) genotypes in Myanmar. Methods: A total of 1333 peripheral blood samples were collected from four different border cities of Myanmar. The anti-HCV antibody-positive serum samples were identified. HCV was genotyped by reverse transcriptase polymerase chain reaction, direct DNA sequencing and phylogenetic analysis on the partial core genome. Results: The overall prevalence of HCV infection was 11.6{\%} (154/1333). Regionally, it was 13.5{\%} (47/349) in the north-eastern city, 12.8{\%} (64/501) in the north-western city, 4.2{\%} (16/380) in the southern city and 26.2{\%} (27/103) in the western city. HCV was genotyped in 145/154 (94.2{\%}) samples. Genotype 6 was the most prevalent genotype in this study (71/145, 49{\%}), followed by genotype 3 (57/145, 39.3{\%}), genotype 1 (16/145, 11{\%}), and genotype 2 (1/145, 0.7{\%}). Genotype 6 was mostly found in the northern cities and genotype 3 in the southern and western cities of Myanmar. Multiple HCV genotypes/subtypes were successfully characterized as 1a, 1b, 2a, 3a, 3b, 6m, 6n, and a new 6 subtype. Among them, subtype 6n was the most predominant subtype (38.6{\%}), followed by subtype 3b (29.7{\%}), 3a (9.6{\%}), 6m (9{\%}), 1b (6.9{\%}), 1a (4.1{\%}), new 6 subtype (1.4{\%}) and 2a (0.7{\%}). Subtype 6n was more widely distributed in the northern cities whereas subtype 3b was more common in the western city. The newly discovered genotype 6 subtype was from the northern cities. Conclusions: The results indicate there are regional differences of HCV genotype distribution in Myanmar. There is a distinct geographic variation from other South-East Asian countries in terms of the existence of the new genotype 6 subtype.",
keywords = "DNA sequencing, Genotype, HepatitisC virus, Myanmar, Phylogenetic analysis",
author = "Lwin, {Aye Aye} and Toshiyuki Shinji and Myo Khin and Ne Win and Mikako Obika and Shigeru Okada and Norio Koide",
year = "2007",
month = "5",
doi = "10.1111/j.1872-034X.2007.00053.x",
language = "English",
volume = "37",
pages = "337--345",
journal = "Hepatology Research",
issn = "1386-6346",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Hepatitis C virus genotype distribution in Myanmar

T2 - Predominance of genotype 6 and existence of new genotype 6 subtype

AU - Lwin, Aye Aye

AU - Shinji, Toshiyuki

AU - Khin, Myo

AU - Win, Ne

AU - Obika, Mikako

AU - Okada, Shigeru

AU - Koide, Norio

PY - 2007/5

Y1 - 2007/5

N2 - Aim: This study was performed to determine the prevalence and distribution of hepatitis C virus (HCV) genotypes in Myanmar. Methods: A total of 1333 peripheral blood samples were collected from four different border cities of Myanmar. The anti-HCV antibody-positive serum samples were identified. HCV was genotyped by reverse transcriptase polymerase chain reaction, direct DNA sequencing and phylogenetic analysis on the partial core genome. Results: The overall prevalence of HCV infection was 11.6% (154/1333). Regionally, it was 13.5% (47/349) in the north-eastern city, 12.8% (64/501) in the north-western city, 4.2% (16/380) in the southern city and 26.2% (27/103) in the western city. HCV was genotyped in 145/154 (94.2%) samples. Genotype 6 was the most prevalent genotype in this study (71/145, 49%), followed by genotype 3 (57/145, 39.3%), genotype 1 (16/145, 11%), and genotype 2 (1/145, 0.7%). Genotype 6 was mostly found in the northern cities and genotype 3 in the southern and western cities of Myanmar. Multiple HCV genotypes/subtypes were successfully characterized as 1a, 1b, 2a, 3a, 3b, 6m, 6n, and a new 6 subtype. Among them, subtype 6n was the most predominant subtype (38.6%), followed by subtype 3b (29.7%), 3a (9.6%), 6m (9%), 1b (6.9%), 1a (4.1%), new 6 subtype (1.4%) and 2a (0.7%). Subtype 6n was more widely distributed in the northern cities whereas subtype 3b was more common in the western city. The newly discovered genotype 6 subtype was from the northern cities. Conclusions: The results indicate there are regional differences of HCV genotype distribution in Myanmar. There is a distinct geographic variation from other South-East Asian countries in terms of the existence of the new genotype 6 subtype.

AB - Aim: This study was performed to determine the prevalence and distribution of hepatitis C virus (HCV) genotypes in Myanmar. Methods: A total of 1333 peripheral blood samples were collected from four different border cities of Myanmar. The anti-HCV antibody-positive serum samples were identified. HCV was genotyped by reverse transcriptase polymerase chain reaction, direct DNA sequencing and phylogenetic analysis on the partial core genome. Results: The overall prevalence of HCV infection was 11.6% (154/1333). Regionally, it was 13.5% (47/349) in the north-eastern city, 12.8% (64/501) in the north-western city, 4.2% (16/380) in the southern city and 26.2% (27/103) in the western city. HCV was genotyped in 145/154 (94.2%) samples. Genotype 6 was the most prevalent genotype in this study (71/145, 49%), followed by genotype 3 (57/145, 39.3%), genotype 1 (16/145, 11%), and genotype 2 (1/145, 0.7%). Genotype 6 was mostly found in the northern cities and genotype 3 in the southern and western cities of Myanmar. Multiple HCV genotypes/subtypes were successfully characterized as 1a, 1b, 2a, 3a, 3b, 6m, 6n, and a new 6 subtype. Among them, subtype 6n was the most predominant subtype (38.6%), followed by subtype 3b (29.7%), 3a (9.6%), 6m (9%), 1b (6.9%), 1a (4.1%), new 6 subtype (1.4%) and 2a (0.7%). Subtype 6n was more widely distributed in the northern cities whereas subtype 3b was more common in the western city. The newly discovered genotype 6 subtype was from the northern cities. Conclusions: The results indicate there are regional differences of HCV genotype distribution in Myanmar. There is a distinct geographic variation from other South-East Asian countries in terms of the existence of the new genotype 6 subtype.

KW - DNA sequencing

KW - Genotype

KW - HepatitisC virus

KW - Myanmar

KW - Phylogenetic analysis

UR - http://www.scopus.com/inward/record.url?scp=34247238854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247238854&partnerID=8YFLogxK

U2 - 10.1111/j.1872-034X.2007.00053.x

DO - 10.1111/j.1872-034X.2007.00053.x

M3 - Article

C2 - 17441806

AN - SCOPUS:34247238854

VL - 37

SP - 337

EP - 345

JO - Hepatology Research

JF - Hepatology Research

SN - 1386-6346

IS - 5

ER -