Hepatic triglyceride lipase plays an essential role in changing the lipid metabolism in genotype 1b hepatitis C virus replicon cells and hepatitis C patients

Yoshiyasu Shinohara, Kento Imajo, Masato Yoneda, Wataru Tomeno, Yuji Ogawa, Koji Fujita, Hiroyuki Kirikoshi, Junichiro Takahashi, Kengo Funakoshi, Masanori Ikeda, Nobuyuki Kato, Atsushi Nakajima, Satoru Saito

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aim: Recently, several studies have shown the existence of associations between lipoprotein profiles and hepatitis C virus (HCV), although only a limited amount of information is available about the mechanisms underlying the changes in the lipoprotein profiles associated with HCV. In this study, we investigated the association between lipoprotein profile, classified according to the particle size, and lipoprotein metabolism. Methods: We used four kinds of cells for this experiment; full-length genome HCV RNA replicon cells (OR6), sub-genomic HCV RNA replicon cells (sO), and OR6c cells and sOc cells, which were the same cell lines treated with interferon-α. The triglyceride (TG) levels in the lipoprotein subclasses of the culture medium were measured by high-performance liquid chromatography. The mRNA expression levels of several molecules associated with lipoprotein metabolism were measured in the OR6, OR6c, sO and sOc cells. To confirm some of the results obtained using the in vitro system, liver biopsy samples obtained from the patients were also examined. Results: The content of TG in the large low-density lipoprotein (LDL) and medium LDL in the culture medium was increased only in the OR6 cells. The hepatic triglyceride lipase (HTGL) mRNA expression levels were lower in the OR6 cells than in the OR6c cells (P

Original languageEnglish
Pages (from-to)1190-1198
Number of pages9
JournalHepatology Research
Volume43
Issue number11
DOIs
Publication statusPublished - Nov 2013

Fingerprint

Replicon
Hepatitis C
Lipase
Lipid Metabolism
Hepacivirus
Genotype
Lipoproteins
Liver
LDL Lipoproteins
Culture Media
Triglycerides
RNA
Messenger RNA
Particle Size
Interferons
High Pressure Liquid Chromatography
Genome
Biopsy
Cell Line

Keywords

  • Hepatic triglyceride lipase
  • Hepatitis C virus
  • High-performance liquid chromatography
  • Lipoprotein metabolism
  • Replicon cell

ASJC Scopus subject areas

  • Hepatology
  • Infectious Diseases

Cite this

Hepatic triglyceride lipase plays an essential role in changing the lipid metabolism in genotype 1b hepatitis C virus replicon cells and hepatitis C patients. / Shinohara, Yoshiyasu; Imajo, Kento; Yoneda, Masato; Tomeno, Wataru; Ogawa, Yuji; Fujita, Koji; Kirikoshi, Hiroyuki; Takahashi, Junichiro; Funakoshi, Kengo; Ikeda, Masanori; Kato, Nobuyuki; Nakajima, Atsushi; Saito, Satoru.

In: Hepatology Research, Vol. 43, No. 11, 11.2013, p. 1190-1198.

Research output: Contribution to journalArticle

Shinohara, Y, Imajo, K, Yoneda, M, Tomeno, W, Ogawa, Y, Fujita, K, Kirikoshi, H, Takahashi, J, Funakoshi, K, Ikeda, M, Kato, N, Nakajima, A & Saito, S 2013, 'Hepatic triglyceride lipase plays an essential role in changing the lipid metabolism in genotype 1b hepatitis C virus replicon cells and hepatitis C patients', Hepatology Research, vol. 43, no. 11, pp. 1190-1198. https://doi.org/10.1111/hepr.12072
Shinohara, Yoshiyasu ; Imajo, Kento ; Yoneda, Masato ; Tomeno, Wataru ; Ogawa, Yuji ; Fujita, Koji ; Kirikoshi, Hiroyuki ; Takahashi, Junichiro ; Funakoshi, Kengo ; Ikeda, Masanori ; Kato, Nobuyuki ; Nakajima, Atsushi ; Saito, Satoru. / Hepatic triglyceride lipase plays an essential role in changing the lipid metabolism in genotype 1b hepatitis C virus replicon cells and hepatitis C patients. In: Hepatology Research. 2013 ; Vol. 43, No. 11. pp. 1190-1198.
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