Abstract
We investigated whether intraoperative infusion of fat emulsion could suppress postoperative endotoxemia and improve energy status in the transplanted liver graft using a swine orthotopic liver transplantation (OLT) model. Intraoperative free fatty acid (FFA) concentration, hepatic FFA clearance, serum hyaluronic acid levels, ATP content and hepatic energy charge (HEC) in liver grafts, postreperfusional endotoxin levels and recipient outcome were compared between a fat emulsion-treated group (LCT- treated group, n = 6) and a saline infused-group (control group, n = 7). Independent of massive surgical stress and administration of heparin, FFA concentration was significantly elevated in the LCT-treated group (P = 0.003). Since FFA clearance, ATP contents and HEC were also increased in the LCT-treated group (P = 0.024, 0.006, 0.005, respectively), intraoperative LCT-treatment was shown to increase FFA concentration and improve energy status of liver grafts. Because there were no significant differences in postoperative hyaluronic acid levels, infused fat emulsion (0.1 g/kg per hour) prevented endothelial cell injury. Significant improvement of postoperative endotoxemia was obtained at 1 and 2 h after reperfusion (P = 0.01 and 0.003, respectively). Energy booster effect and antiseptic effect led to prolonged survival of recipients in the LCT-treated group (28.5 ± 13.3 days, P = 0.013 vs. control). We concluded that the hepatic energy booster effect and the lipo-modulatory effect on postreperfusional endotoxemia caused by intraoperative infusion of fat emulsion may be of great use in human liver transplantation. (C) 2000 Elsevier Science Ireland Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 72-82 |
| Number of pages | 11 |
| Journal | Hepatology Research |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 2000 |
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Keywords
- ATP
- Clearance
- Endotoxin
- Energy charge
- Free fatty acid
- Lipid emulsion
- Liver transplantation
ASJC Scopus subject areas
- Gastroenterology
Cite this
Hepatic energy booster effect and lipo-modulatory effect on postreperfusional endotoxemia by intraoperative lipid infusion in porcine liver transplantation. / Yagi, Takahito; Ishikawa, Takashi; Oishi, Masahiro; Matsuda, Hiroaki; Endo, Akira; Matsukawa, Hiroyoshi; Nakao, Atsunori; Okada, Yutaka; Sadamori, Hiroshi; Inagaki, Masaru; Takakura, Norihisa; Tanaka, Noriaki.
In: Hepatology Research, Vol. 17, No. 1, 03.2000, p. 72-82.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hepatic energy booster effect and lipo-modulatory effect on postreperfusional endotoxemia by intraoperative lipid infusion in porcine liver transplantation
AU - Yagi, Takahito
AU - Ishikawa, Takashi
AU - Oishi, Masahiro
AU - Matsuda, Hiroaki
AU - Endo, Akira
AU - Matsukawa, Hiroyoshi
AU - Nakao, Atsunori
AU - Okada, Yutaka
AU - Sadamori, Hiroshi
AU - Inagaki, Masaru
AU - Takakura, Norihisa
AU - Tanaka, Noriaki
PY - 2000/3
Y1 - 2000/3
N2 - We investigated whether intraoperative infusion of fat emulsion could suppress postoperative endotoxemia and improve energy status in the transplanted liver graft using a swine orthotopic liver transplantation (OLT) model. Intraoperative free fatty acid (FFA) concentration, hepatic FFA clearance, serum hyaluronic acid levels, ATP content and hepatic energy charge (HEC) in liver grafts, postreperfusional endotoxin levels and recipient outcome were compared between a fat emulsion-treated group (LCT- treated group, n = 6) and a saline infused-group (control group, n = 7). Independent of massive surgical stress and administration of heparin, FFA concentration was significantly elevated in the LCT-treated group (P = 0.003). Since FFA clearance, ATP contents and HEC were also increased in the LCT-treated group (P = 0.024, 0.006, 0.005, respectively), intraoperative LCT-treatment was shown to increase FFA concentration and improve energy status of liver grafts. Because there were no significant differences in postoperative hyaluronic acid levels, infused fat emulsion (0.1 g/kg per hour) prevented endothelial cell injury. Significant improvement of postoperative endotoxemia was obtained at 1 and 2 h after reperfusion (P = 0.01 and 0.003, respectively). Energy booster effect and antiseptic effect led to prolonged survival of recipients in the LCT-treated group (28.5 ± 13.3 days, P = 0.013 vs. control). We concluded that the hepatic energy booster effect and the lipo-modulatory effect on postreperfusional endotoxemia caused by intraoperative infusion of fat emulsion may be of great use in human liver transplantation. (C) 2000 Elsevier Science Ireland Ltd.
AB - We investigated whether intraoperative infusion of fat emulsion could suppress postoperative endotoxemia and improve energy status in the transplanted liver graft using a swine orthotopic liver transplantation (OLT) model. Intraoperative free fatty acid (FFA) concentration, hepatic FFA clearance, serum hyaluronic acid levels, ATP content and hepatic energy charge (HEC) in liver grafts, postreperfusional endotoxin levels and recipient outcome were compared between a fat emulsion-treated group (LCT- treated group, n = 6) and a saline infused-group (control group, n = 7). Independent of massive surgical stress and administration of heparin, FFA concentration was significantly elevated in the LCT-treated group (P = 0.003). Since FFA clearance, ATP contents and HEC were also increased in the LCT-treated group (P = 0.024, 0.006, 0.005, respectively), intraoperative LCT-treatment was shown to increase FFA concentration and improve energy status of liver grafts. Because there were no significant differences in postoperative hyaluronic acid levels, infused fat emulsion (0.1 g/kg per hour) prevented endothelial cell injury. Significant improvement of postoperative endotoxemia was obtained at 1 and 2 h after reperfusion (P = 0.01 and 0.003, respectively). Energy booster effect and antiseptic effect led to prolonged survival of recipients in the LCT-treated group (28.5 ± 13.3 days, P = 0.013 vs. control). We concluded that the hepatic energy booster effect and the lipo-modulatory effect on postreperfusional endotoxemia caused by intraoperative infusion of fat emulsion may be of great use in human liver transplantation. (C) 2000 Elsevier Science Ireland Ltd.
KW - ATP
KW - Clearance
KW - Endotoxin
KW - Energy charge
KW - Free fatty acid
KW - Lipid emulsion
KW - Liver transplantation
UR - http://www.scopus.com/inward/record.url?scp=0034010405&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034010405&partnerID=8YFLogxK
U2 - 10.1016/S1386-6346(99)00056-X
DO - 10.1016/S1386-6346(99)00056-X
M3 - Article
AN - SCOPUS:0034010405
VL - 17
SP - 72
EP - 82
JO - Hepatology Research
JF - Hepatology Research
SN - 1386-6346
IS - 1
ER -