Heparanase regulates esophageal keratinocyte differentiation through nuclear translocation and heparan sulfate cleavage

Masahiko Kobayashi, Yoshio Naomoto, Tetsuji Nobuhisa, Takaomi Okawa, Munenori Takaoka, Yasuhiro Shirakawa, Tomoki Yamatsuji, Junji Matsuoka, Takaaki Mizushima, Hironori Matsuura, Motowo Nakajima, Hiroshi Nakagawa, Anil Rustgi, Noriaki Tanaka

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39 Citations (Scopus)

Abstract

Heparanase is an endo-β-glucuronidase that specifically cleaves heparan sulfate (HS) chains. Heparanase is involved in the process of metastasis and angiogenesis through the degradation of HS chains of the extracellular matrix and cell surface. Recently, we demonstrated that heparanase was localized in the cell nucleus of normal esophageal epithelium and esophageal cancer, and that its expression was correlated with cell differentiation. However, the nuclear function of heparanase remains unknown. To elucidate the role of heparanase in esophageal epithelial differentiation, primary human esophageal cells were grown in monolayer as well as organotypic cultures, and cell differentiation was induced. Expression of heparanase, HS, involucrin, and p27 was determined by immunostaining and Western blotting. SF4, a novel pharmacological inhibitor, was used to specifically inhibit heparanase activity. Upon esophageal cell differentiation, heparanase was translocated from the cytoplasm to the nucleus. Such translocation of heparanase appeared to be associated with the degradation of HS chains in the nucleus and changes in the expression of keratinocyte differentiation markers such as p27 and involucrin, whose induction was inhibited by SF4. Furthermore, these in vitro observations agreed with the expression pattern of heparanase, HS, involucrin, cytokeratin 13, and p27 in normal esophageal epithelium. Nuclear translocation of heparanase and its catalytic cleavage of HS may play a critical role in the differentiation of esophageal epithelial cells. Our study provides a novel insight into the role of heparanase in an essential differentiation process.

Original languageEnglish
Pages (from-to)235-243
Number of pages9
JournalDifferentiation
Volume74
Issue number5
DOIs
Publication statusPublished - Jun 2006

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Keywords

  • Differentiation
  • Esophageal keratinocyte
  • Heparan sulfate
  • Heparanase
  • Nuclear translocation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

Cite this

Kobayashi, M., Naomoto, Y., Nobuhisa, T., Okawa, T., Takaoka, M., Shirakawa, Y., Yamatsuji, T., Matsuoka, J., Mizushima, T., Matsuura, H., Nakajima, M., Nakagawa, H., Rustgi, A., & Tanaka, N. (2006). Heparanase regulates esophageal keratinocyte differentiation through nuclear translocation and heparan sulfate cleavage. Differentiation, 74(5), 235-243. https://doi.org/10.1111/j.1432-0436.2006.00072.x