Heparanase localization during palatogenesis in mice

Azumi Hirata, Kentaro Katayama, Takehito Tsuji, Nagato Natsume, Toshio Sugahara, Yuichi Koga, Kazufumi Takano, Yoshinori Otsuki, Hiroaki Nakamura

Research output: Contribution to journalArticle

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Abstract

Palatogenesis is directed by epithelial-mesenchymal interactions and results partly from remodeling of the extracellular matrix (ECM) of the palatal shelves. Here, we assessed heparanase distribution in developing mouse palates. No heparanase was observed in the vertically oriented palatal shelves in early stages of palate formation. As palate formation progressed, the palatal shelves were reorganized and arranged horizontally above the tongue, and heparanase localized to the epithelial cells of these shelves. When the palatal bilateral shelves first made contact, the heparanase localized to epithelial cells at the tips of shelves. Later in fusing palatal shelves, the cells of the medial epithelial seam (MES) were labeled with intense heparanase signal. In contrast, the basement membrane heparan sulfate (HS) was scarcely observed in the palatal shelves in contact. Moreover, perlecan labeling was sparse in the basement membrane of the MES, on which laminin and type IV collagen were observed. Moreover, we assessed the distribution of matrix metalloproteinase- (MMP-) 9, MMP-2, and MMP-3 in developing mouse palates and these MMPs were observed in the MES. Our findings indicated that heparanase was important for palate formation because it mediated degradation of the ECM of palatal shelves. Heparanase may, in concert with other proteases, participate in the regression of the MES.

Original languageEnglish
Article number760236
JournalBioMed Research International
Volume2013
DOIs
Publication statusPublished - 2013

Fingerprint

Palate
Epithelial Cells
Basement Membrane
Extracellular Matrix
Matrix Metalloproteinase 3
Heparitin Sulfate
Collagen Type IV
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Laminin
heparanase
Matrix Metalloproteinases
Tongue
Labeling
Peptide Hydrolases
Degradation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Hirata, A., Katayama, K., Tsuji, T., Natsume, N., Sugahara, T., Koga, Y., ... Nakamura, H. (2013). Heparanase localization during palatogenesis in mice. BioMed Research International, 2013, [760236]. https://doi.org/10.1155/2013/760236

Heparanase localization during palatogenesis in mice. / Hirata, Azumi; Katayama, Kentaro; Tsuji, Takehito; Natsume, Nagato; Sugahara, Toshio; Koga, Yuichi; Takano, Kazufumi; Otsuki, Yoshinori; Nakamura, Hiroaki.

In: BioMed Research International, Vol. 2013, 760236, 2013.

Research output: Contribution to journalArticle

Hirata, A, Katayama, K, Tsuji, T, Natsume, N, Sugahara, T, Koga, Y, Takano, K, Otsuki, Y & Nakamura, H 2013, 'Heparanase localization during palatogenesis in mice', BioMed Research International, vol. 2013, 760236. https://doi.org/10.1155/2013/760236
Hirata, Azumi ; Katayama, Kentaro ; Tsuji, Takehito ; Natsume, Nagato ; Sugahara, Toshio ; Koga, Yuichi ; Takano, Kazufumi ; Otsuki, Yoshinori ; Nakamura, Hiroaki. / Heparanase localization during palatogenesis in mice. In: BioMed Research International. 2013 ; Vol. 2013.
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