Heparanase expression correlates with invasion and poor prognosis in gastric cancers

Munenori Takaoka, Yoshio Naomoto, Takaomi Ohkawa, Hirokazu Uetsuka, Yasuhiro Shirakawa, Futoshi Uno, Toshiyoshi Fujiwara, Mehmet Gunduz, Hitoshi Nagatsuka, Motowo Nakajima, Noriaki Tanaka, Minoru Haisa

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Degradation of basement membrane and extracellular matrix structures are important features of the metastatic process of malignant tumors. Human heparanase degrades heparan sulfate proteoglycans, which represent the main components of basement membranes and the extracellular matrix. Because of the role of heparanase in tumor invasion and metastasis, we examined heparanase expression in primary gastric cancers and in cell lines derived from gastric cancers by immunohistochemistry and RT-PCR, respectively. Four of seven gastric cancer cell lines showed heparanase mRNA expression by RT-PCR. Heparanase protein was detected in both the cytoplasm and the nucleus of heparanase mRNA-positive cells by immunohistochemical staining. Heparanase expression was confirmed in 35 (79.5%) of 44 gastric tumor samples by immunohistochemical staining. However, no or weak heparanase expression was detected in normal gastric mucosa. In situ hybridization showed that the mRNA expression pattern of heparanase was similar to that of the protein, suggesting that increased expression of the heparanase protein at the invasive front was caused by an increase of heparanase mRNA in tumor cells. Analysis of the clinicopathologic features showed stronger heparanase expression in cases of huge growing tumors, extensive invasion to lymph vessels, and regional lymph node metastasis. In gastric cancer, patients with heparanase expression showed significantly poorer prognosis than those without such expression (p = 0.006). In conclusion, our findings suggest that high expression of heparanase in gastric cancer is a strong predictor of poor survival.

Original languageEnglish
Pages (from-to)613-622
Number of pages10
JournalLaboratory Investigation
Volume83
Issue number5
Publication statusPublished - May 1 2003

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Stomach Neoplasms
Messenger RNA
Neoplasms
heparanase
Basement Membrane
Extracellular Matrix
Staining and Labeling
Neoplasm Metastasis
Cell Line
Polymerase Chain Reaction
Heparan Sulfate Proteoglycans
Proteins
Lymph
Gastric Mucosa
In Situ Hybridization
Stomach
Cytoplasm
Lymph Nodes
Immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Takaoka, M., Naomoto, Y., Ohkawa, T., Uetsuka, H., Shirakawa, Y., Uno, F., ... Haisa, M. (2003). Heparanase expression correlates with invasion and poor prognosis in gastric cancers. Laboratory Investigation, 83(5), 613-622.

Heparanase expression correlates with invasion and poor prognosis in gastric cancers. / Takaoka, Munenori; Naomoto, Yoshio; Ohkawa, Takaomi; Uetsuka, Hirokazu; Shirakawa, Yasuhiro; Uno, Futoshi; Fujiwara, Toshiyoshi; Gunduz, Mehmet; Nagatsuka, Hitoshi; Nakajima, Motowo; Tanaka, Noriaki; Haisa, Minoru.

In: Laboratory Investigation, Vol. 83, No. 5, 01.05.2003, p. 613-622.

Research output: Contribution to journalArticle

Takaoka, M, Naomoto, Y, Ohkawa, T, Uetsuka, H, Shirakawa, Y, Uno, F, Fujiwara, T, Gunduz, M, Nagatsuka, H, Nakajima, M, Tanaka, N & Haisa, M 2003, 'Heparanase expression correlates with invasion and poor prognosis in gastric cancers', Laboratory Investigation, vol. 83, no. 5, pp. 613-622.
Takaoka M, Naomoto Y, Ohkawa T, Uetsuka H, Shirakawa Y, Uno F et al. Heparanase expression correlates with invasion and poor prognosis in gastric cancers. Laboratory Investigation. 2003 May 1;83(5):613-622.
Takaoka, Munenori ; Naomoto, Yoshio ; Ohkawa, Takaomi ; Uetsuka, Hirokazu ; Shirakawa, Yasuhiro ; Uno, Futoshi ; Fujiwara, Toshiyoshi ; Gunduz, Mehmet ; Nagatsuka, Hitoshi ; Nakajima, Motowo ; Tanaka, Noriaki ; Haisa, Minoru. / Heparanase expression correlates with invasion and poor prognosis in gastric cancers. In: Laboratory Investigation. 2003 ; Vol. 83, No. 5. pp. 613-622.
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