Abstract
Delirium occurs in 23% of sepsis patients, in which pro-inflammatory cytokines and nitric oxide are suggested to be involved. However, in animal experiments, even a subseptic dose of lipopolysaccharide (LPS) injection induces both pro-inflammatory cytokines and inducible nitric oxide synthase in the brain, suggesting that the brain oxidative reaction can be induced in the subseptic condition. Then, we evaluated the changes of heme oxygenase-1 (HO-1), a sensitive oxidative marker, as well as interleukin (IL)-1β, IL-6, and inductible nitric oxide synthase (iNOS) mRNA in the hypothalamus and hippocampus of rats using real-time PCR after peripheral injection of LPS (2.0 mg/kg). As a result, these four kinds of mRNAs were induced significantly in both areas after LPS injection. These results suggest that peripheral inflammation induces an oxidative reaction in the brain, even if the inflammation is not lethal. It is also considered that several pathways are involved in brain HO-1 induction.
| Original language | English |
|---|---|
| Pages (from-to) | 663-667 |
| Number of pages | 5 |
| Journal | Neuropsychiatric Disease and Treatment |
| Volume | 4 |
| Issue number | 3 |
| Publication status | Published - 2008 |
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Keywords
- Heme oxygenase-1
- Hippocampus
- Hypothalamus
- Interleukin-1β
- Interleukin-6
- Lipopolysaccharide
ASJC Scopus subject areas
- Psychiatry and Mental health
- Biological Psychiatry
Cite this
Heme oxygenase-I induction in the brain during lipopolysaccharide-induced acute inflammation. / Maeda, Shigeru; Nakatsuka, Ichiro; Hayashi, Yukiko; Higuchi, Hitoshi; Shimada, Masahiko; Miyawaki, Takuya.
In: Neuropsychiatric Disease and Treatment, Vol. 4, No. 3, 2008, p. 663-667.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heme oxygenase-I induction in the brain during lipopolysaccharide-induced acute inflammation
AU - Maeda, Shigeru
AU - Nakatsuka, Ichiro
AU - Hayashi, Yukiko
AU - Higuchi, Hitoshi
AU - Shimada, Masahiko
AU - Miyawaki, Takuya
PY - 2008
Y1 - 2008
N2 - Delirium occurs in 23% of sepsis patients, in which pro-inflammatory cytokines and nitric oxide are suggested to be involved. However, in animal experiments, even a subseptic dose of lipopolysaccharide (LPS) injection induces both pro-inflammatory cytokines and inducible nitric oxide synthase in the brain, suggesting that the brain oxidative reaction can be induced in the subseptic condition. Then, we evaluated the changes of heme oxygenase-1 (HO-1), a sensitive oxidative marker, as well as interleukin (IL)-1β, IL-6, and inductible nitric oxide synthase (iNOS) mRNA in the hypothalamus and hippocampus of rats using real-time PCR after peripheral injection of LPS (2.0 mg/kg). As a result, these four kinds of mRNAs were induced significantly in both areas after LPS injection. These results suggest that peripheral inflammation induces an oxidative reaction in the brain, even if the inflammation is not lethal. It is also considered that several pathways are involved in brain HO-1 induction.
AB - Delirium occurs in 23% of sepsis patients, in which pro-inflammatory cytokines and nitric oxide are suggested to be involved. However, in animal experiments, even a subseptic dose of lipopolysaccharide (LPS) injection induces both pro-inflammatory cytokines and inducible nitric oxide synthase in the brain, suggesting that the brain oxidative reaction can be induced in the subseptic condition. Then, we evaluated the changes of heme oxygenase-1 (HO-1), a sensitive oxidative marker, as well as interleukin (IL)-1β, IL-6, and inductible nitric oxide synthase (iNOS) mRNA in the hypothalamus and hippocampus of rats using real-time PCR after peripheral injection of LPS (2.0 mg/kg). As a result, these four kinds of mRNAs were induced significantly in both areas after LPS injection. These results suggest that peripheral inflammation induces an oxidative reaction in the brain, even if the inflammation is not lethal. It is also considered that several pathways are involved in brain HO-1 induction.
KW - Heme oxygenase-1
KW - Hippocampus
KW - Hypothalamus
KW - Interleukin-1β
KW - Interleukin-6
KW - Lipopolysaccharide
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M3 - Article
VL - 4
SP - 663
EP - 667
JO - Neuropsychiatric Disease and Treatment
JF - Neuropsychiatric Disease and Treatment
SN - 1176-6328
IS - 3
ER -