Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period

Y. Katayama, N. Mahmut, H. Takimoto, Yoshinobu Maeda, T. Yano, K. Kojima, T. Azuma, M. Hara, K. Imajyo, S. Takahashi, T. Kai, Y. Ohno, T. Miyamoto, K. Nagafuji, K. Matsue, K. Takenaka, T. Teshima, K. Shinagawa, F. Ishimaru, E. Omoto & 1 others M. Harada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Despite the therapeutic efficacy of allogeneic bone marrow transplantation (allo-BMT), circulating hematopoietic progenitor cells after bone marrow transplantation have not been well characterized. In the present study, we focused on these 'post-transplant circulating progenitor cells (PTCPC)' which may be on their way to bone marrow. We analyzed the number of myeloid progenitor cells (CFU-GM) per 10 ml of peripheral blood (PB) on days 0 (just before transplantation), 1 (8-15 h after the completion of transplantation), 2, 3, 5, 7, 10, 14, 17, 21, 28 and 35 after allo-BMT in five transplant patients using a standard methylcellulose assay. In addition, high proliferative potential colony-forming cells (HPP-CFC) of the harvested donor bone marrow (BM) and day 1 PB of recipients were assayed in five patients. The origin of HPP-CFC from day 1 PB was analyzed by polymerase chain reaction of a DNA region containing a variable number of tandem repeats. The replating potential of these HPP-CFC was evaluated by a secondary colony assay. The proportion of CD38(negative) cells among CD34+ cells in the harvested BM and day 1 PB was evaluated by two-color flow cytometric analysis. The number of CFU-GM on day 1 ranged from 6 to 73/10 ml PB, and became undetectable on day 5. The reappearance of PTCPC was observed on day 14, along with hematopoietic recovery. The proportion of HPP-CFC among myeloid colonies from day 1 PB was significantly higher than that from harvested BM (44.3 ± 10.4% vs 11.3 ± 2.1%, respectively, n = 5, P = 0.0030). These HPP-CFC from day 1 PB were confirmed to be of donor origin. More than 90% of these HPP-CFC had replating potential. Two-color flow cytometric analysis revealed that the proportion of CD34+CD38(negative) cells was significantly higher in day 1 PB than in the harvested BM (61.0 ± 16.5% vs 9.3 ± 3.5%, respectively, n = 7, P = 0.0002). These observations suggest that both primitive and committed transplanted myeloid progenitor cells may circulate in the very early period following allo-BMT.

Original languageEnglish
Pages (from-to)659-665
Number of pages7
JournalBone Marrow Transplantation
Volume23
Issue number7
Publication statusPublished - 1999

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Hematopoietic Stem Cells
Bone Marrow
Tissue Donors
Transplants
Bone Marrow Transplantation
Homologous Transplantation
Myeloid Progenitor Cells
Granulocyte-Macrophage Progenitor Cells
Stem Cells
Color
Transplantation
Minisatellite Repeats
Methylcellulose
Myeloid Cells
Polymerase Chain Reaction
DNA

Keywords

  • Allo-BMT
  • CD34CD38 cells
  • Circulating progenitor cells
  • HPP-CFC

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period. / Katayama, Y.; Mahmut, N.; Takimoto, H.; Maeda, Yoshinobu; Yano, T.; Kojima, K.; Azuma, T.; Hara, M.; Imajyo, K.; Takahashi, S.; Kai, T.; Ohno, Y.; Miyamoto, T.; Nagafuji, K.; Matsue, K.; Takenaka, K.; Teshima, T.; Shinagawa, K.; Ishimaru, F.; Omoto, E.; Harada, M.

In: Bone Marrow Transplantation, Vol. 23, No. 7, 1999, p. 659-665.

Research output: Contribution to journalArticle

Katayama, Y, Mahmut, N, Takimoto, H, Maeda, Y, Yano, T, Kojima, K, Azuma, T, Hara, M, Imajyo, K, Takahashi, S, Kai, T, Ohno, Y, Miyamoto, T, Nagafuji, K, Matsue, K, Takenaka, K, Teshima, T, Shinagawa, K, Ishimaru, F, Omoto, E & Harada, M 1999, 'Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period', Bone Marrow Transplantation, vol. 23, no. 7, pp. 659-665.
Katayama, Y. ; Mahmut, N. ; Takimoto, H. ; Maeda, Yoshinobu ; Yano, T. ; Kojima, K. ; Azuma, T. ; Hara, M. ; Imajyo, K. ; Takahashi, S. ; Kai, T. ; Ohno, Y. ; Miyamoto, T. ; Nagafuji, K. ; Matsue, K. ; Takenaka, K. ; Teshima, T. ; Shinagawa, K. ; Ishimaru, F. ; Omoto, E. ; Harada, M. / Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period. In: Bone Marrow Transplantation. 1999 ; Vol. 23, No. 7. pp. 659-665.
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T1 - Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period

AU - Katayama, Y.

AU - Mahmut, N.

AU - Takimoto, H.

AU - Maeda, Yoshinobu

AU - Yano, T.

AU - Kojima, K.

AU - Azuma, T.

AU - Hara, M.

AU - Imajyo, K.

AU - Takahashi, S.

AU - Kai, T.

AU - Ohno, Y.

AU - Miyamoto, T.

AU - Nagafuji, K.

AU - Matsue, K.

AU - Takenaka, K.

AU - Teshima, T.

AU - Shinagawa, K.

AU - Ishimaru, F.

AU - Omoto, E.

AU - Harada, M.

PY - 1999

Y1 - 1999

N2 - Despite the therapeutic efficacy of allogeneic bone marrow transplantation (allo-BMT), circulating hematopoietic progenitor cells after bone marrow transplantation have not been well characterized. In the present study, we focused on these 'post-transplant circulating progenitor cells (PTCPC)' which may be on their way to bone marrow. We analyzed the number of myeloid progenitor cells (CFU-GM) per 10 ml of peripheral blood (PB) on days 0 (just before transplantation), 1 (8-15 h after the completion of transplantation), 2, 3, 5, 7, 10, 14, 17, 21, 28 and 35 after allo-BMT in five transplant patients using a standard methylcellulose assay. In addition, high proliferative potential colony-forming cells (HPP-CFC) of the harvested donor bone marrow (BM) and day 1 PB of recipients were assayed in five patients. The origin of HPP-CFC from day 1 PB was analyzed by polymerase chain reaction of a DNA region containing a variable number of tandem repeats. The replating potential of these HPP-CFC was evaluated by a secondary colony assay. The proportion of CD38(negative) cells among CD34+ cells in the harvested BM and day 1 PB was evaluated by two-color flow cytometric analysis. The number of CFU-GM on day 1 ranged from 6 to 73/10 ml PB, and became undetectable on day 5. The reappearance of PTCPC was observed on day 14, along with hematopoietic recovery. The proportion of HPP-CFC among myeloid colonies from day 1 PB was significantly higher than that from harvested BM (44.3 ± 10.4% vs 11.3 ± 2.1%, respectively, n = 5, P = 0.0030). These HPP-CFC from day 1 PB were confirmed to be of donor origin. More than 90% of these HPP-CFC had replating potential. Two-color flow cytometric analysis revealed that the proportion of CD34+CD38(negative) cells was significantly higher in day 1 PB than in the harvested BM (61.0 ± 16.5% vs 9.3 ± 3.5%, respectively, n = 7, P = 0.0002). These observations suggest that both primitive and committed transplanted myeloid progenitor cells may circulate in the very early period following allo-BMT.

AB - Despite the therapeutic efficacy of allogeneic bone marrow transplantation (allo-BMT), circulating hematopoietic progenitor cells after bone marrow transplantation have not been well characterized. In the present study, we focused on these 'post-transplant circulating progenitor cells (PTCPC)' which may be on their way to bone marrow. We analyzed the number of myeloid progenitor cells (CFU-GM) per 10 ml of peripheral blood (PB) on days 0 (just before transplantation), 1 (8-15 h after the completion of transplantation), 2, 3, 5, 7, 10, 14, 17, 21, 28 and 35 after allo-BMT in five transplant patients using a standard methylcellulose assay. In addition, high proliferative potential colony-forming cells (HPP-CFC) of the harvested donor bone marrow (BM) and day 1 PB of recipients were assayed in five patients. The origin of HPP-CFC from day 1 PB was analyzed by polymerase chain reaction of a DNA region containing a variable number of tandem repeats. The replating potential of these HPP-CFC was evaluated by a secondary colony assay. The proportion of CD38(negative) cells among CD34+ cells in the harvested BM and day 1 PB was evaluated by two-color flow cytometric analysis. The number of CFU-GM on day 1 ranged from 6 to 73/10 ml PB, and became undetectable on day 5. The reappearance of PTCPC was observed on day 14, along with hematopoietic recovery. The proportion of HPP-CFC among myeloid colonies from day 1 PB was significantly higher than that from harvested BM (44.3 ± 10.4% vs 11.3 ± 2.1%, respectively, n = 5, P = 0.0030). These HPP-CFC from day 1 PB were confirmed to be of donor origin. More than 90% of these HPP-CFC had replating potential. Two-color flow cytometric analysis revealed that the proportion of CD34+CD38(negative) cells was significantly higher in day 1 PB than in the harvested BM (61.0 ± 16.5% vs 9.3 ± 3.5%, respectively, n = 7, P = 0.0002). These observations suggest that both primitive and committed transplanted myeloid progenitor cells may circulate in the very early period following allo-BMT.

KW - Allo-BMT

KW - CD34CD38 cells

KW - Circulating progenitor cells

KW - HPP-CFC

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