Hematopoietic cells derived from cancer stem cells generated from mouse induced pluripotent stem cells

Ghmkin Hassan, Said M. Afify, Neha Nair, Kazuki Kumon, Amira Osman, Juan Du, Hager Mansour, Hagar A.Abu Quora, Hend M. Nawara, Ayano Satoh, Maram H. Zahra, Nobuhiro Okada, Akimasa Seno, Masaharu Seno

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to differentiate into hematopoietic cells. First, the primary cells were isolated from malignant tumors that were formed by the CSCs. Non-adherent cells (NACs) that arose from adherent cells were collected and their viability, as well as the morphology and expression of hematopoietic cell markers, were analyzed. Moreover, NACs were injected into the tail vein of busulfan conditioned Balb/c nude mice. Finally, CSCs were induced to differentiate to macrophages while using IL3 and SCF. The round nucleated NACs were found to be viable, positive for hematopoietic lineage markers and CD34, and expressed hematopoietic markers, just like homing to the bone marrow. When NACs were injected into mice, Wright–Giemsa staining showed that the number of white blood cells got higher than those in the control mice after four weeks. CSCs also showed the ability to differentiate toward macrophages. CSCs were demonstrated to have the potential to provide progenies with hematopoietic markers, morphology, and homing ability to the bone marrow, which could give new insight into the tumor microenvironment according to the plasticity of CSCs.

Original languageEnglish
Article number82
JournalCancers
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 2020

Keywords

  • Cancer stem cells differentiation
  • Hematopoietic cells
  • Induced pluripotent stem cells
  • Tumor microenvironment

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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