Helicobacter pylori induces pepsinogen secretion by rat gastric cells in culture via a cAMP signal pathway.

H. X. Jiang, H. Pu, N. H. Huh, Kenji Yokota, K. Oguma, M. Namba

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Infection with Helicobater pylori (H. pylori) is associated with various stomach diseases such as chronic gastritis, peptic ulcer, and gastric carcinoma. In order to investigate the mechanisms of enhanced production of pepsinogen by H. pylori in cultured rat gastric cells that have the potential to produce pepsinogen, secretion and synthesis of pepsinogen in the cells exposed to H. pylori extract were determined by measuring the hydrolysis of hemoglobin. Various drugs were used to study the mechanisms of effects of H. pylori on the cells. Exposure of the gastric cells to H. pylori extract caused a significant increase in pepsinogen secretion into the culture medium within 30-180 min in a dose-dependent manner, accompanied by a significant increase in pepsinogen synthesis in the gastric cells after 60 min of incubation. Heat treatment of the H. pylori sonicate at 100 degrees C for 10 min completely abolished the stimulatory effect of H. pylori on pepsinogen secretion. 2',3'-Dideoxyadenosine (50 microM), a specific adenylate cyclase inhibitor, abolished the effect of H. pylori-induced pepsinogen secretion. Puromycin (10 microg/ml), a protein synthesis inhibitor, and nicorandil (0.1 mM), a specific intracellular calcium antagonist, reduced the H. pylori-induced pepsinogen secretion by 37% (p

Original languageEnglish
Pages (from-to)625-629
Number of pages5
JournalInternational Journal of Molecular Medicine
Volume7
Issue number6
Publication statusPublished - Jun 2001

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Pepsinogen A
Pylorus
Helicobacter pylori
Signal Transduction
Stomach
Cell Culture Techniques
Dideoxyadenosine
Nicorandil
Stomach Diseases
Puromycin
Protein Synthesis Inhibitors
Gastritis
Peptic Ulcer
Culture Media
Hemoglobins
Hydrolysis
Hot Temperature
Calcium
Carcinoma

ASJC Scopus subject areas

  • Genetics

Cite this

Helicobacter pylori induces pepsinogen secretion by rat gastric cells in culture via a cAMP signal pathway. / Jiang, H. X.; Pu, H.; Huh, N. H.; Yokota, Kenji; Oguma, K.; Namba, M.

In: International Journal of Molecular Medicine, Vol. 7, No. 6, 06.2001, p. 625-629.

Research output: Contribution to journalArticle

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AU - Namba, M.

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