Helicobacter pylori heat-shock protein 60 induces production of the pro-inflammatory cytokine IL8 in monocytic cells

Song Nan Lin, Kiyoshi Ayada, Ying Zhao, Kenji Yokota, Ryuta Takenaka, Hiroyuki Okada, Rui Kan, Shyunji Hayashi, Motowo Mizuno, Yoshikazu Hirai, Yoshihito Fujinami, Keiji Oguma

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Abstract

Interleukin 8 (IL8) is usually produced in both epithelial and monocytic cells during bacterial infections, causing inflammation. Helicobacter pylori induces production of IL8 from gastric epithelial cells via its cag pathogenicity island (cag PAI) system, LPS and outer-membrane protein. In some bacteria, heat-shock protein 60 (HSP60) also elicits a strong pro-inflammatory response in cells of the innate immune system. Three recombinant H. pylori HSP60 (rHSP60) proteins of different sizes were produced and one of these was used to raise two monoclonal antibodies (2E7 and 7B5). IL8 production was found to be induced in cultured monocytic cells treated with H. pylori cells or rHSP60 proteins, as measured by ELISA, and the amount of IL8 produced was dose-dependent. Pre-incubation of H. pylori cells or rHSP60 preparations with the antibody 2E7 significantly inhibited IL8 production from monocytic cells. These results indicated that HSP60 is closely associated with IL8 production in monocytic cells.

Original languageEnglish
Pages (from-to)225-233
Number of pages9
JournalJournal of Medical Microbiology
Volume54
Issue number3
DOIs
Publication statusPublished - Mar 2005

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ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Lin, S. N., Ayada, K., Zhao, Y., Yokota, K., Takenaka, R., Okada, H., Kan, R., Hayashi, S., Mizuno, M., Hirai, Y., Fujinami, Y., & Oguma, K. (2005). Helicobacter pylori heat-shock protein 60 induces production of the pro-inflammatory cytokine IL8 in monocytic cells. Journal of Medical Microbiology, 54(3), 225-233. https://doi.org/10.1099/jmm.0.45871-0