Helices F-G are important for the substrate specificities of CYP3A7

Nao Torimoto, Itsuko Ishii, Ken Ichi Toyama, Masayuki Hata, Kanako Tanaka, Hitoshi Shimomura, Hiroyoshi Nakamura, Noritaka Ariyoshi, Shigeru Ohmori, Mitsukazu Kitada

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

CYP3A7 is a member of the human CYP3A family and a major form of P450 expressed in human fetal livers. Although CYP3A7 shares nearly 90% base sequence with CYP3A4, CYP3A7 shows striking functional differences in the catalytic preference for several substrates, such as dehydroepiandrosterone (DHEA) or dehydroepiandrosterone 3-sulfate (DHEA-3S). First, to clarify the reason for the differences between CYP3A7 and CYP3A4, a homology model of CYP3A7 was constructed using the CYP3A4 crystal structure. Because these two structures were similar, four kinds of chimeric enzymes were constructed to determine which sequences are important for exhibiting the characteristics of CYP3A7. The results of kinetic analysis of DHEA and DHEA-3S 16α-hydroxylations by CYP3A7, CYP3A4, and CYP3A chimeras suggested that the amino acid residues from Leu210 to Glu279 were important to express the specificity for substrates as CYP3A7. This region was on the F and G helices of the modeled CYP3A7. Furthermore, to assess which amino acid in this sequence is important for the substrate specificity of CYP3A7, a one-point mutation of CYP3A7 to CYP3A4 was made by site-directed mutagenesis. The mutants of K224T and K244E had lost DHEA and DHEA-3S 16α-hydroxylation activities. The mutants also greatly decreased the metabolism of testosterone, erythromycin, nevirapine, and triazolam relative to those activities of CYP3A7 wild-type enzyme. From these results, it is expected that CYP3A7 can recognize specific substrates using the lysines in F-G loops.

Original languageEnglish
Pages (from-to)484-492
Number of pages9
JournalDrug Metabolism and Disposition
Volume35
Issue number3
DOIs
Publication statusPublished - Mar 2007
Externally publishedYes

Fingerprint

Cytochrome P-450 CYP3A
Substrate Specificity
Dehydroepiandrosterone
Substrates
Hydroxylation
Triazolam
Nevirapine
Amino Acids
Mutagenesis
Dehydroepiandrosterone Sulfate
Erythromycin
Enzymes
Site-Directed Mutagenesis
G-substrate
Point Mutation
Metabolism
Liver
Sulfates
Lysine
Testosterone

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Torimoto, N., Ishii, I., Toyama, K. I., Hata, M., Tanaka, K., Shimomura, H., ... Kitada, M. (2007). Helices F-G are important for the substrate specificities of CYP3A7. Drug Metabolism and Disposition, 35(3), 484-492. https://doi.org/10.1124/dmd.106.011304

Helices F-G are important for the substrate specificities of CYP3A7. / Torimoto, Nao; Ishii, Itsuko; Toyama, Ken Ichi; Hata, Masayuki; Tanaka, Kanako; Shimomura, Hitoshi; Nakamura, Hiroyoshi; Ariyoshi, Noritaka; Ohmori, Shigeru; Kitada, Mitsukazu.

In: Drug Metabolism and Disposition, Vol. 35, No. 3, 03.2007, p. 484-492.

Research output: Contribution to journalArticle

Torimoto, N, Ishii, I, Toyama, KI, Hata, M, Tanaka, K, Shimomura, H, Nakamura, H, Ariyoshi, N, Ohmori, S & Kitada, M 2007, 'Helices F-G are important for the substrate specificities of CYP3A7', Drug Metabolism and Disposition, vol. 35, no. 3, pp. 484-492. https://doi.org/10.1124/dmd.106.011304
Torimoto N, Ishii I, Toyama KI, Hata M, Tanaka K, Shimomura H et al. Helices F-G are important for the substrate specificities of CYP3A7. Drug Metabolism and Disposition. 2007 Mar;35(3):484-492. https://doi.org/10.1124/dmd.106.011304
Torimoto, Nao ; Ishii, Itsuko ; Toyama, Ken Ichi ; Hata, Masayuki ; Tanaka, Kanako ; Shimomura, Hitoshi ; Nakamura, Hiroyoshi ; Ariyoshi, Noritaka ; Ohmori, Shigeru ; Kitada, Mitsukazu. / Helices F-G are important for the substrate specificities of CYP3A7. In: Drug Metabolism and Disposition. 2007 ; Vol. 35, No. 3. pp. 484-492.
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